Inzitari Rosanna, Cabras Tiziana, Pisano Elisabetta, Fanali Chiara, Manconi Barbara, Scarano Emanuele, Fiorita Antonella, Paludetti Gaetano, Manni Armando, Nemolato Sonia, Faa Gavino, Castagnola Massimo, Messana Irene
Istituto di Biochimica e di Biochimica Clinica, Università Cattolica and Istituto per la Chimica del Riconoscimento Molecolare, CNR, Istituto Scientifico Internazionale (ISI) Paolo VI, Roma, Italy.
J Sep Sci. 2009 Jan;32(1):57-63. doi: 10.1002/jssc.200800496.
Thymosin beta(4) (Tbeta(4)), its sulfoxide, and thymosin beta(10 )(Tbeta(10)) were detected in human saliva and identified by different strategies based on RP HPLC coupled to electrospray multidimensional IT MS. Tbeta(4 )was almost always detected in whole saliva, its sulfoxide sporadically, Tbeta(10) rarely. Tbeta(4) was undetectable in parotid saliva and less concentrated in submandibular/sublingual saliva than in whole saliva. Analysis of gingival crevicular fluid revealed high relative amounts of Tbeta(4), Tbeta(4) sulfoxide, and Tbeta(10) in all the samples. Tbeta(4) mean concentration was 200 times higher in crevicular fluid (20 micromol/L, N = 9) than in whole saliva (0.1 micromol/L, N = 9). Crevicular fluid concentration of Tbeta(4 )(ca. 5% represented by its sulfoxide) and beta(10 )significantly correlated (r = 0.856; N = 9), and their ratio was about 5. A significant correlation was also observed between Tbeta(4 )concentrations in whole saliva and gingival crevicular fluid (r = 0.738; N = 9). Immunohistochemical analysis of the major salivary glands showed that immunoreactivity for Tbeta(4) is restricted to ductal cells, with minor degree of focal positivity in some acinar cells. On the whole, results indicate that gingival sulcus is a main, although not the sole, source for oral Tbeta(4 )and Tbeta(10).
在人类唾液中检测到了胸腺素β(4)（Tβ(4)）、其亚砜以及胸腺素β(10)（Tβ(10)），并基于反相高效液相色谱联用电喷雾多维离子阱质谱，通过不同策略对其进行了鉴定。Tβ(4)几乎总能在全唾液中检测到，其亚砜偶尔能检测到，Tβ(10)则很少检测到。腮腺唾液中未检测到Tβ(4)，且下颌下腺/舌下腺唾液中的Tβ(4)浓度低于全唾液。龈沟液分析显示，所有样本中Tβ(4)、Tβ(4)亚砜和Tβ(10)的相对含量都很高。龈沟液中Tβ(4)的平均浓度（20微摩尔/升，N = 9）比全唾液（0.1微摩尔/升，N = 9）高200倍。龈沟液中Tβ(4)（约5%由其亚砜代表）和β(10)的浓度显著相关（r = 0.856；N = 9），其比例约为5。全唾液和龈沟液中Tβ(4)的浓度之间也观察到显著相关性（r = 0.738；N = 9）。对主要唾液腺的免疫组织化学分析表明，Tβ(4)的免疫反应性仅限于导管细胞，一些腺泡细胞有轻微程度的局灶性阳性。总体而言，结果表明龈沟是口腔中Tβ(4)和Tβ(10)的主要来源，尽管不是唯一来源。
