National Research Council (CNR), Institute for Nanostructured Materials (ISMN), Via P. Gobetti 101, 40129 Bologna, Italy.
Dipartimento di Chimica "G. Ciamician", Alma Mater Studiorum-Università di Bologna, via F. Selmi 2, 40126 Bologna, Italy.
Biomacromolecules. 2021 Aug 9;22(8):3357-3365. doi: 10.1021/acs.biomac.1c00463. Epub 2021 Jul 17.
A green biocompatible route for the deposition and simultaneous assembly, by pH increment, of collagen/chitin composites was proposed. Both assembled and unassembled samples with different collagen/chitin ratios were synthesized, maintaining the β-chitin polymorph. The first set showed a microfibrous organization with compositional submicron homogeneity. The second set presented a nanohomogeneous composition based on collagen nanoaggregates and chitin nanofibrils. The sets were tested as scaffolds for fibroblast growth (NIH-3T3) to study the influence of composition and assembly. In the unassembled scaffolds, the positive influence of collagen on cell growth mostly worn out in 48 h, while the addition of chitin enhanced this effect for over 72 h. The assembled samples showed higher viability at 24 h but a less positive effect on viability along the time. This work highlighted critical aspects of the influence that composition and assembly has on fibroblast growth, a knowledge worth exploiting in scaffold design and preparation.
提出了一种绿色的生物相容性方法，通过 pH 值增加，同时进行胶原蛋白/壳聚糖复合材料的沉积和组装。合成了具有不同胶原蛋白/壳聚糖比例的组装和未组装样品，保持β-壳聚糖多晶型。第一组显示出具有组成亚微米均匀性的微纤维组织。第二组基于胶原蛋白纳米聚集体和壳聚糖纳米纤维呈现纳米均匀的组成。将两组作为成纤维细胞生长（NIH-3T3）的支架进行测试，以研究组成和组装的影响。在未组装的支架中，胶原蛋白对细胞生长的积极影响在 48 小时后基本消失，而壳聚糖的添加则使这种影响持续超过 72 小时。组装样品在 24 小时时具有更高的存活率，但随着时间的推移对存活率的影响不太积极。这项工作强调了组成和组装对成纤维细胞生长影响的关键方面，这一知识在支架设计和制备中值得开发利用。
