Donnadio Anna, Paul Geo, Barbalinardo Marianna, Ambrogi Valeria, Pettinacci Gabriele, Posati Tamara, Bisio Chiara, Vivani Riccardo, Nocchetti Morena
Department of Pharmaceutical Sciences, University of Perugia, Via Del Liceo 1, 06123 Perugia, Italy.
CEMIN-Centro di Eccellenza Materiali Innovativi Nanostrutturati, University of Perugia, Via Elce di Sotto 8, 06123 Perugia, Italy.
Nanomaterials (Basel). 2023 Feb 15;13(4):742. doi: 10.3390/nano13040742.
Different amounts of sodium-alendronate (ALN) were loaded into layered zirconium phosphates of alpha and gamma type (αZP and γZP) by means of topotactic exchange reactions of phosphate with ALN. In order to extend the exchange process to the less accessible interlayer regions, ALN solutions were contacted with colloidal dispersions of the layered solids previously exfoliated in single sheets by means of intercalation reaction of propylamine (for αZP) or acetone (for γZP). The ALN loading degree was determined by liquid P-nuclear magnetic resonance (NMR) and inductively coupled plasma (ICP), and it was reported as ALN/Zr molar ratios (Rs). The maximum R obtained for γZP was 0.34, while αZP was able to load a higher amount of ALN, reaching Rs equal to 1. The synthesized compounds were characterized by X-ray powder diffractometry, scanning electron microscopy (SEM), solid-state NMR, and infrared spectroscopy. The way the grafted organo-phosphonate groups were bonded to the layers of the host structure was suggested. The effect of ZP derivatives was assessed on cell proliferation, and the results showed that after 7 days of incubation, none of the samples showed a decrease in cell proliferation.
通过磷酸盐与阿仑膦酸钠（ALN）的拓扑交换反应，将不同量的阿仑膦酸钠（ALN）负载到α型和γ型层状磷酸锆（αZP和γZP）中。为了将交换过程扩展到较难到达的层间区域，使ALN溶液与通过丙胺（用于αZP）或丙酮（用于γZP）的插层反应预先剥离成单张薄片的层状固体的胶体分散体接触。通过液体磷核磁共振（NMR）和电感耦合等离子体（ICP）测定ALN负载量，并将其报告为ALN/Zr摩尔比（Rs）。γZP获得的最大Rs为0.34，而αZP能够负载更高量的ALN，Rs达到1。通过X射线粉末衍射、扫描电子显微镜（SEM）、固态NMR和红外光谱对合成的化合物进行了表征。提出了接枝的有机膦酸酯基团与主体结构层结合的方式。评估了ZP衍生物对细胞增殖的影响，结果表明，孵育7天后，没有一个样品显示细胞增殖减少。
