Chongqing Rapeseed Engineering Research Center, College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, China.
Academy of Agricultural Sciences, Southwest University, Chongqing 400715, China.
Int J Mol Sci. 2021 Jul 5;22(13):7215. doi: 10.3390/ijms22137215.
The allotetraploid species (mustard) is grown worldwide as oilseed and vegetable crops; the yellow seed-color trait is particularly important for oilseed crops. Here, to examine the factors affecting seed coat color, we performed a metabolic and transcriptomic analysis of yellow- and dark-seeded . seeds. In this study, we identified 236 compounds, including 31 phenolic acids, 47 flavonoids, 17 glucosinolates, 38 lipids, 69 other hydroxycinnamic acid compounds, and 34 novel unknown compounds. Of these, 36 compounds (especially epicatechin and its derivatives) accumulated significantly different levels during the development of yellow- and dark-seeded . . In addition, the transcript levels of , ,, and were closely associated with changes to epicatechin and its derivatives during seed development, implicating this pathway in the seed coat color determinant in . . Furthermore, we found numerous variations of sequences in the genes that may be associated with the stability of seed coat color in . , . and . , which might have undergone functional differentiation during polyploidization in the species. The results provide valuable information for understanding the accumulation of metabolites in the seed coat color of and lay a foundation for exploring the underlying mechanism.
异源四倍体物种 （芥菜）作为油料作物和蔬菜作物在全球范围内种植；黄色种皮颜色性状对油料作物尤为重要。在这里，为了研究影响种皮颜色的因素，我们对黄色和深色种皮的 进行了代谢组学和转录组学分析。在这项研究中，我们鉴定了 236 种化合物，包括 31 种酚酸、47 种类黄酮、17 种硫代葡萄糖苷、38 种脂质、69 种其他羟基肉桂酸化合物和 34 种新的未知化合物。其中，36 种化合物（特别是表儿茶素及其衍生物）在黄色和深色种皮 的发育过程中积累水平显著不同。此外， 、 、 和 的转录水平与种子发育过程中表儿茶素及其衍生物的变化密切相关，表明该途径参与了 种皮颜色决定因子的形成。此外，我们还发现 基因中的许多序列变异可能与 、 、 和 中种皮颜色的稳定性有关。 和 可能在 物种的多倍化过程中发生了功能分化。这些结果为理解 种皮颜色中代谢物的积累提供了有价值的信息，并为探索其潜在机制奠定了基础。
