Dairy Science Department, National Research Center, Dokki, Cairo, Egypt.
Microbiology Department, Faculty of Science, Ain Shams University, Cairo, Egypt.
Arch Microbiol. 2021 Oct;203(8):5017-5028. doi: 10.1007/s00203-021-02484-3. Epub 2021 Jul 20.
Kidney stones composed of oxalate are a significant health problem. It has been suggested that modification of the intestinal microbiota to reduce the amount of oxalate in the digestive system could be an effective treatment. There have been several studies into the use of lactic acid bacteria for the degradation of intestinal oxalates. We isolated 88 lactic acid bacteria strains from a range of dairy products, and screened for their ability to degrade oxalate. Using the oxalate-degrading Enzymatic Activity Index and the viable cell counts, five strains of Lactobacillus fermentum and two strains of Lactobacillus gastricus were identified as having strong oxalate degradation abilities, and were further investigated. All seven strains were able to tolerate acid (pH 4 and 3), bile salts (0.3%), phenol (0.3%), and to produce exopolysaccharides. They were resistant to a wide range of antibiotics. Among these strains, Lactobacillus fermentum NRAMJ5 and Lactobacillus gastricus NRAMJ2 were, therefore, good candidates as probiotics for managing hyperoxaluria.
由草酸形成的肾结石是一个严重的健康问题。有人提出，通过改变肠道微生物群来减少消化系统中的草酸含量可能是一种有效的治疗方法。已经有一些关于使用乳酸菌来降解肠道草酸的研究。我们从各种乳制品中分离出 88 株乳酸菌，并筛选其降解草酸的能力。使用草酸降解酶活性指数和活菌计数，我们鉴定出 5 株发酵乳杆菌和 2 株胃乳杆菌具有很强的草酸降解能力，并进一步进行了研究。这 7 株菌均能耐受酸性（pH 值 4 和 3）、胆盐（0.3%）、苯酚（0.3%），并能产生胞外多糖。它们对多种抗生素有耐药性。在这些菌株中，发酵乳杆菌 NRAMJ5 和胃乳杆菌 NRAMJ2 因此是作为管理高草酸尿症的益生菌的良好候选菌株。
