Cell Therapy Institute, Spinal Cord Injury and Tissue Regeneration Center Salzburg (SCI - TReCS), Paracelsus Medical University (PMU), Salzburg, Austria.
Cell Therapy Institute, Spinal Cord Injury and Tissue Regeneration Center Salzburg (SCI - TReCS), Paracelsus Medical University (PMU), Salzburg, Austria.
Adv Drug Deliv Rev. 2021 Sep;176:113872. doi: 10.1016/j.addr.2021.113872. Epub 2021 Jul 17.
Rigorous measures are required to cope with the advance of extracellular vesicle (EV) research, from 183 studies published in 2012 to 2,309 studies published in 2020. The International Society for Extracellular Vesicles (ISEV) proposed Minimal Information for Studies of Extracellular Vesicles (MISEV) guidelines in 2014, updated in 2018, for assuring and improving EV research quality. We performed a systematic review using a text mining approach to assess adherence to MISEV criteria. A keyword search was conducted in 5,093 accessible publications over the period 2012-2020 and analyzed the methodology used for EV isolation and characterization. We found a significant improvement over the years particularly regarding EV characterization where recent papers used a higher number of methods and EV markers to check for quantity and purity. Interestingly, we also found that EV papers using more methods and EV markers were cited more frequently. Papers citing MISEV criteria were more prone to use a higher number of characterization methods. We therefore established a concise checklist summarizing MISEV criteria to support EV researchers towards reaching the highest standards in the field.
需要采取严格的措施来应对细胞外囊泡（EV）研究的进展，从 2012 年发表的 183 项研究到 2020 年发表的 2309 项研究。国际细胞外囊泡学会（ISEV）于 2014 年提出了细胞外囊泡研究的最低信息要求（MISEV）指南，并于 2018 年进行了更新，以确保和提高 EV 研究质量。我们使用文本挖掘方法进行了系统评价，以评估对 MISEV 标准的遵守情况。在 2012-2020 年期间，我们对 5093 篇可获取的文献进行了关键字搜索，并分析了用于 EV 分离和表征的方法。我们发现，随着时间的推移，这方面有了显著的改善，特别是在 EV 特征方面，最近的论文使用了更多的方法和 EV 标志物来检查数量和纯度。有趣的是，我们还发现使用更多方法和 EV 标志物的 EV 论文被引用的频率更高。引用 MISEV 标准的论文更倾向于使用更多的特征描述方法。因此，我们建立了一个简明的清单，总结了 MISEV 标准，以支持 EV 研究人员在该领域达到最高标准。
