Van Dycke Jana, Neyts Johan, Rocha-Pereira Joana
Department of Microbiology and Immunology, Rega Institute for Medical Research, Laboratory of Virology and Chemotherapy, KU Leuven - University of Leuven, Leuven, Belgium.
Bio Protoc. 2018 May 5;8(9):e2831. doi: 10.21769/BioProtoc.2831.
Human norovirus is the most common cause of acute gastroenteritis worldwide, resulting in estimated mortality of ~210,000 each year, of whom most are children under the age of five. However, norovirus can infect people of all age groups. There is a risk of prolonged infection in children, the elderly and patients who are immunocompromised. To study the inhibition of persistent norovirus replication by small molecule antivirals , we used a murine norovirus CR6 strain (MNV.CR6). We demonstrated earlier that efficient small molecules can reduce viral shedding in the stool of infected mice. Here we present how to generate the MNV.CR6 virus stock, infect type I and II interferon receptor knockout AG129 mice via oral gavage, administer antivirals to mice, and quantify viral genome copies in the stool of these mice.
人诺如病毒是全球急性胃肠炎最常见的病因，估计每年导致约21万人死亡，其中大多数是五岁以下儿童。然而，诺如病毒可感染所有年龄组的人群。儿童、老年人和免疫功能低下患者存在持续感染的风险。为了研究小分子抗病毒药物对诺如病毒持续复制的抑制作用，我们使用了鼠诺如病毒CR6株（MNV.CR6）。我们之前证明，有效的小分子可以减少感染小鼠粪便中的病毒排出。在此，我们介绍如何制备MNV.CR6病毒储备液，通过口服灌胃感染I型和II型干扰素受体敲除的AG129小鼠，给小鼠施用抗病毒药物，以及定量这些小鼠粪便中的病毒基因组拷贝数。
