Pei Na, Hao Zhiyan, Wang Sen, Pan Binglei, Fang Ao, Kang Jianfeng, Li Dichen, He Jiankang, Wang Ling
State Key Laboratory for Manufacturing System Engineering, Xi'an Jiaotong University, 710054, Xi'an, Shaanxi, China.
School of Mechanical Engineering, Xi'an Jiaotong University, 710054, Xi'an, ShanXi, China.
Int J Bioprint. 2021 Jun 15;7(3):359. doi: 10.18063/ijb.v7i3.359. eCollection 2021.
The pathological research and drug development of brain diseases require appropriate brain models. Given the complex, layered structure of the cerebral cortex, as well as the constraints on the medical ethics and the inaccuracy of animal models, it is necessary to construct a brain-like model . In this study, we designed and built integrated three-dimensional (3D) printing equipment for cell printing/culture, which can guarantee cell viability in the printing process and provide the equipment foundation for manufacturing the layered structures with gradient distribution of pore size. Based on this printing equipment, to achieve the purpose of printing the layered structures with multiple materials, we conducted research on the performance of bio-inks with different compositions and optimized the printing process. By extruding and stacking materials, we can print the layered structure with the uniform distribution of cells and the gradient distribution of pore sizes. Finally, we can accurately print a structure with 30 layers. The line width (resolution) of the printed monolayer structure was about 478 mm, the forming accuracy can reach 97.24%, and the viability of cells in the printed structure is as high as 94.5%.
脑部疾病的病理研究和药物开发需要合适的脑模型。鉴于大脑皮层复杂的分层结构，以及医学伦理的限制和动物模型的不准确性，构建类脑模型是必要的。在本研究中，我们设计并制造了用于细胞打印/培养的集成三维（3D）打印设备，该设备能够在打印过程中保证细胞活力，并为制造具有孔径梯度分布的分层结构提供设备基础。基于此打印设备，为实现用多种材料打印分层结构的目的，我们对不同成分的生物墨水性能进行了研究，并优化了打印工艺。通过挤压和堆叠材料，我们能够打印出细胞均匀分布且孔径呈梯度分布的分层结构。最终，我们能够精确打印出具有30层的结构。打印的单层结构的线宽（分辨率）约为478毫米，成型精度可达97.24%，打印结构中细胞的活力高达94.5%。
