Ishiguro S, Nishio A, Miyao N, Morikawa Y, Takeno K, Yanagiya I
Department of Veterinary Pharmacology, Faculty of Agriculture, Kagoshima University, Japan.
Nihon Yakurigaku Zasshi. 1987 Sep;90(3):141-6. doi: 10.1254/fpj.90.141.
When young Wistar rats (body wt. 50 g) were maintained on a magnesium-deficient diet (0.001% Mg) for eight days, the splenic weight and histamine content increased about 2-fold and 30-fold, respectively, compared with those of the control rats. There was no significant difference in the number of splenic mast cells between the magnesium-deficient and control rats. More neutrophilic, eosinophilic and basophilic granular cells were found in the spleen cells isolated from the magnesium-deficient rats than in those from the control rats. Of the isolated cells from magnesium-deficient rats, 7.6% were basophilic granular cells; however, no basophilic granular cells were observed in the spleen cells isolated from the control rats. In the cytochemical study, the yellowish fluorochrome formed by the interaction of o-phthalaldehyde and histamine was found in basophilic granular cells. These results suggest that the increase in the histamine content of the spleen of magnesium-deficient rats is related to the increased number of basophilic granular cells.
当幼年Wistar大鼠（体重50克）在缺镁饮食（0.001%镁）中饲养8天时，与对照大鼠相比，脾脏重量和组胺含量分别增加了约2倍和30倍。缺镁大鼠和对照大鼠的脾脏肥大细胞数量没有显著差异。与对照大鼠分离的脾细胞相比，缺镁大鼠分离的脾细胞中发现更多的嗜中性、嗜酸性和嗜碱性粒细胞。在缺镁大鼠分离的细胞中，7.6%是嗜碱性粒细胞；然而，在对照大鼠分离的脾细胞中未观察到嗜碱性粒细胞。在细胞化学研究中，在嗜碱性粒细胞中发现了由邻苯二甲醛与组胺相互作用形成的淡黄色荧光染料。这些结果表明，缺镁大鼠脾脏中组胺含量的增加与嗜碱性粒细胞数量的增加有关。
