Saleh Samar R, Masry Asmaa M, Ghareeb Doaa A, Newairy Al-Sayeda A, Sheta Eman, Maher Adham M
Department of Biochemistry, Faculty of Science, Alexandria University, Alexandria, 21511, Egypt.
Bioscreening and Preclinical Trial Lab, Biochemistry Department, Faculty of Science, Alexandria University, Alexandria, Egypt.
Sci Rep. 2021 Jul 21;11(1):14872. doi: 10.1038/s41598-021-94058-y.
Date pits are nutritious by-products, containing high levels of indigestible carbohydrates and polyphenols. To maximize the biological effects of the active ingredients, the hard shell of the polysaccharide must be degraded. Therefore, the current study aimed to assess the protective potentials of date pits extract (DP) and fungal degraded date pits extract (FDDP) against scopolamine (SCO)-induced neurodegeneration in male rats. Date pits were subjected to fungal degradation and extraction, followed by the measurement of phytochemicals and free radical scavenging activities. Forty-two adult Sprague-Dawley male rats were divided into seven groups: three control groups administered with either saline, DP or FDDP; four groups with neurodegeneration receiving SCO (ip 2 mg/kg/day, SCO group) with no treatment, SCO with DP (oral 100 mg/kg/day, DP + SCO group), SCO with FDDP (oral, 100 mg/kg/day, FDDP + SCO group), and SCO with donepezil (DON, oral, 2.25 mg/kg/day, DON + SCO group). The treatment duration was 28 days, and in the last 14 days, SCO was administered daily. Morris water maze test, acetylcholine esterase activity, oxidative stress, markers of inflammation and amyloidogenesis, and brain histopathology were assessed.
椰枣核是营养丰富的副产品，含有大量难以消化的碳水化合物和多酚。为了使活性成分的生物学效应最大化，必须降解多糖的硬壳。因此，本研究旨在评估椰枣核提取物（DP）和真菌降解椰枣核提取物（FDDP）对东莨菪碱（SCO）诱导的雄性大鼠神经退行性变的保护潜力。对椰枣核进行真菌降解和提取，随后测定植物化学物质和自由基清除活性。将42只成年Sprague-Dawley雄性大鼠分为7组：3个对照组分别给予生理盐水、DP或FDDP；4个神经退行性变组，一组接受SCO（腹腔注射2mg/kg/天，SCO组）且不进行治疗，一组SCO联合DP（口服100mg/kg/天，DP+SCO组），一组SCO联合FDDP（口服，100mg/kg/天，FDDP+SCO组），一组SCO联合多奈哌齐（DON，口服，2.25mg/kg/天，DON+SCO组）。治疗持续28天，在最后14天，每天给予SCO。评估了Morris水迷宫试验、乙酰胆碱酯酶活性、氧化应激、炎症和淀粉样蛋白生成标志物以及脑组织病理学。
