Bakour Meryem, El Menyiy Nawal, El Ghouizi Asmae, Lyoussi Badiaa
Laboratory of Natural Substances, Pharmacology, Environment, Modeling, Health and Quality of life (SNAMOPEQ), Sidi Mohamed Ben Abdellah University, Fez, Morocco.
Avicenna J Phytomed. 2021 Jul-Aug;11(4):343-352. doi: 10.22038/AJP.2021.17748.
This study aims to shed a new light on pharmacological effects of bee bread as a product of the hive through examination of the effect of itsethyl acetate extract onhyperglycemia, dyslipidemia, and liver dysfunction induced by streptozotocin.
The bee bread ethyl acetate extract was analyzed for total phenolics, flavonoids, and the antioxidant activities using total antioxidant capacity, 2, 2- diphenyl-1-picrylhydrazyl (DPPH), 2, 2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS), and reducing power assays. study was carried out on thirty-six rats divided into control or diabetic rats, received daily for 15 days distilled water (10 ml/kg), or ethyl acetate extract of bee bread (100 mg/kg), or glibenclamide (2.5 mg/kg). The protective effect of bee bread against metabolic changes induced by streptozotocin in Wistar rats, was evaluated by checking the blood glucose levels, lipid profile, atherogenic index, coronary risk index, cardiovascular risk index, body weight and hepatic enzyme markers in normal and diabetic rats. Glibenclamide was used as standard drug to compare the efficacy of bee bread.
The results indicate that bee bread ethyl acetate extract has a high content of phenolics and flavonoids and a strong antioxidant activity. Glycemia, lipid profile and hepatic enzymes were modified in diabetic rats. These modifications were ameliorated after the treatment withbee bread extract which was more potent than glibenclamide.
In summary, ethyl acetate extract of bee bread possesses effective glycemia lowering effects and representsa natural source of new bioactive molecules for future therapy of hyperglycemia, hyperlipidemia and liver dysfunction.
本研究旨在通过检测蜂粮乙酸乙酯提取物对链脲佐菌素诱导的高血糖、血脂异常和肝功能障碍的影响，为蜂粮作为蜂巢产品的药理作用提供新的见解。
采用总抗氧化能力、2,2-二苯基-1-苦基肼（DPPH）、2,2'-联氮-双-（3-乙基苯并噻唑啉-6-磺酸）（ABTS）和还原力测定法，分析蜂粮乙酸乙酯提取物中的总酚、黄酮类化合物和抗氧化活性。对36只大鼠进行研究，将其分为对照组或糖尿病组，连续15天每天给予蒸馏水（10 ml/kg）、蜂粮乙酸乙酯提取物（100 mg/kg）或格列本脲（2.5 mg/kg）。通过检测正常和糖尿病大鼠的血糖水平、血脂谱、致动脉粥样硬化指数、冠状动脉风险指数、心血管风险指数、体重和肝酶标志物，评估蜂粮对Wistar大鼠链脲佐菌素诱导的代谢变化的保护作用。格列本脲用作标准药物以比较蜂粮的疗效。
结果表明，蜂粮乙酸乙酯提取物含有高含量的酚类和黄酮类化合物，具有较强的抗氧化活性。糖尿病大鼠的血糖、血脂谱和肝酶发生了改变。用蜂粮提取物治疗后，这些改变得到改善，且蜂粮提取物比格列本脲更有效。
总之，蜂粮乙酸乙酯提取物具有有效的降血糖作用，是未来治疗高血糖、高血脂和肝功能障碍的新生物活性分子的天然来源。
