角豆蜂蜜水提取物和乙酸乙酯提取物对链脲佐菌素诱导的糖尿病大鼠的抗氧化、降血糖和肝保护作用
Antioxidant, hypoglycemic, and hepatoprotective effect of aqueous and ethyl acetate extract of carob honey in streptozotocin-induced diabetic rats.
作者信息
El-Haskoury Redouan, Al-Waili Noori, El-Hilaly Jaouad, Al-Waili Waili, Lyoussi Badiaa
机构信息
Department of Biology Physiology-Pharmacology and Environmental Health Laboratory, Faculty of Sciences Dhar-Mahraz, Sidi Mohamed Ben Abdallah University, Fez, Morocco.
Department of Scientific Research, New York Medical Care for Nephrology, Richmond Hill, NY 11418, USA.
出版信息
Vet World. 2019 Dec;12(12):1916-1923. doi: 10.14202/vetworld.2019.1916-1923. Epub 2019 Dec 10.
AIM
The aim of the study included the effect of aqueous extract (AE) and ethyl acetate extract (EAE) on blood sugar in diabetic rats and their effects on liver enzymes and lipid panel in control and diabetic rats. Furthermore, the antioxidant activity of the EAE was studied and compared with AE.
MATERIALS AND METHODS
Sugar and antioxidant content of AE and EAE were determined. antioxidant activity of AE and EAE was estimated by 2, 2-diphenyl-1-picrylhydrazyl and ABTS*+ radical scavenging assay, ferric-reducing antioxidant power assay, and total antioxidant assay. To study the effect of the extracts on blood glucose level (BGL), lipid profile, and liver function in non-diabetic and diabetic rats, five groups of six rats each were treated with distilled water, AE, EAE, glibenclamide (GLB), and sucrose for 8 days. Plasma glucose level (PGL), total cholesterol (TC), triglycerides (TG), transaminases (alanine transaminase [ALT] and aspartate transaminase [AST]), and alkaline phosphatase (ALP) were determined. The effect of the interventions on BGL after acute administration also was investigated. Diabetes was induced by streptozotocin injection.
RESULTS
EAE contains significantly lower content of fructose and glucose than AE (p<0.05), and it has no sucrose. AE and EAE exhibited a significant antioxidant activity and high antioxidant content; the antioxidant content was higher in AE than EAE (p<0.05). In diabetic rats, acute treatment by AE increased PGL, while EAE significantly lowered BGL as compared to the untreated diabetic rats. Both interventions significantly decreased BGL as compared to the sucrose treated group in diabetic rats (p<0.05). EAE was more potent than GLB. Sucrose caused 13% increment in BGL after 8 days of induction of diabetes, while AE caused only 1.3% increment. Daily treatment by EAE decreased significantly AST, ALT, ALP, and TC. EAE decreased significantly TC and TG level in diabetic rats in comparison to the untreated diabetic group.
CONCLUSION
The study showed for the 1 time that EAE has more hypoglycemic effect than AE, and both extracts prevent the increment in BGL on day 8 after induction of diabetes observed in the control and sucrose treated group. EAE significantly ameliorated the lipid and liver function disorders induced by diabetes.
目的
本研究的目的包括水提取物（AE）和乙酸乙酯提取物（EAE）对糖尿病大鼠血糖的影响及其对正常和糖尿病大鼠肝酶及血脂指标的影响。此外，还研究了EAE的抗氧化活性并与AE进行比较。
材料与方法
测定AE和EAE的糖分及抗氧化剂含量。通过2,2-二苯基-1-苦基肼自由基清除法、ABTS⁺自由基清除法、铁还原抗氧化能力测定法和总抗氧化能力测定法评估AE和EAE的抗氧化活性。为研究提取物对非糖尿病和糖尿病大鼠血糖水平（BGL）、血脂谱和肝功能的影响，将五组每组六只大鼠分别用蒸馏水、AE、EAE、格列本脲（GLB）和蔗糖处理8天。测定血浆葡萄糖水平（PGL）、总胆固醇（TC）、甘油三酯（TG）、转氨酶（丙氨酸转氨酶[ALT]和天冬氨酸转氨酶[AST]）以及碱性磷酸酶（ALP）。还研究了急性给药后干预措施对BGL的影响。通过注射链脲佐菌素诱导糖尿病。
结果
EAE中果糖和葡萄糖的含量明显低于AE（p<0.05），且不含蔗糖。AE和EAE均表现出显著的抗氧化活性和较高的抗氧化剂含量；AE中的抗氧化剂含量高于EAE（p<0.05）。在糖尿病大鼠中，AE急性治疗可使PGL升高，而与未治疗的糖尿病大鼠相比，EAE可显著降低BGL。与糖尿病大鼠中的蔗糖治疗组相比，两种干预措施均显著降低了BGL（p<0.05）。EAE比GLB更有效。糖尿病诱导8天后，蔗糖使BGL升高了13%，而AE仅使BGL升高了1.3%。EAE每日治疗可显著降低AST、ALT、ALP和TC。与未治疗的糖尿病组相比，EAE显著降低了糖尿病大鼠的TC和TG水平。
结论
该研究首次表明EAE的降血糖作用比AE更强，且两种提取物均能防止在对照组和蔗糖治疗组中观察到的糖尿病诱导后第8天BGL的升高。EAE显著改善了糖尿病引起的脂质和肝功能紊乱。
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