Program in Nanoscience and Technology, Graduate School of Convergence Science and Technology, Seoul National University, Seoul, 08826, Republic of Korea.
Department of Materials Science and Engineering, University of Michigan, Ann Arbor, Michigan 48109, United States.
ACS Sens. 2021 Sep 24;6(9):3170-3175. doi: 10.1021/acssensors.1c01167. Epub 2021 Jul 22.
The necessity of a simple measurement of platelet activation has been increasing in clinical medicine to regulate the proper dose of the antiplatelet drugs for patients having clinical outcomes in acute situations such as angina pectoris, stroke, or peripheral vascular disease or procedures involving angioplasty or coronary thrombolysis. We developed a self-signaling polydiacetylene (PDA) liposome microarray to detect activated platelets from whole blood samples in a single step. A specific antibody, 9F9 antibody, to platelet-bound fibrinogen was selected and conjugated to the PDA liposome microarray to quantify the fibrinogen-bound platelets. The developed PDA liposome-9F9 microarray generated an intense fluorescence signal when activated platelets in whole blood were introduced and also successfully distinguished the reduced platelet activation in the presence of Tirofiban, a model antiplatelet drug. The results of this single-step benchtop assay incorporates simple, sensitive, and rapid attributes that can detect the extent of platelet activation prior to needed clinical procedures.
为了调节抗血小板药物的适当剂量，临床医学中越来越需要对血小板活化进行简单的测量，以针对心绞痛、中风、外周血管疾病或涉及经皮腔内血管成形术或冠状动脉溶栓的临床情况的患者进行适当治疗。我们开发了一种自信号多聚二乙炔（PDA）脂质体微阵列，可一步检测全血样本中的活化血小板。选择针对血小板结合纤维蛋白原的特异性抗体 9F9 抗体，并将其共轭到 PDA 脂质体微阵列上，以定量纤维蛋白原结合的血小板。当将全血中的活化血小板引入开发的 PDA 脂质体-9F9 微阵列中时，该微阵列会产生强烈的荧光信号，并且还成功区分了替罗非班（一种模型抗血小板药物）存在时血小板活化的减少。该一步台式检测的结果具有简单、灵敏和快速的特点，可在需要进行临床操作之前检测血小板活化的程度。
