Alimentary Pharmabiotic Centre, Department of Microbiology, University College Cork, Cork, Ireland.
J Appl Microbiol. 2013 Apr;114(4):1132-46. doi: 10.1111/jam.12105. Epub 2013 Jan 11.
To evaluate the ability of specific carbohydrates, including commercially available products, to support the growth of representatives of two well-known groups of gut commensals, namely lactobacilli and bifidobacteria.
Sixty-eight bacterial strains, representing 29 human-derived lactobacilli and 39 bifidobacteria (both human- and animal-derived), were tested for their ability to metabolize 10 different carbohydrates. Analysis of growth and metabolic activity was performed using a combination of diagnostic parameters, such as final OD600 , final pH, fermentation end products and growth rate.
The data assembled in this study provide significant complementary and comparative information on the growth-promoting properties of a range of carbohydrates, while also investigating interspecies differences between lactobacilli and/or bifidobacteria with regard to their carbohydrate utilization abilities. Galacto-oligosaccharides (GOS) and lactulose were shown to support the most favourable growth characteristics, whereas relatively poor growth of lactobacilli and bifidobacteria was observed on inulin, maltodextrin and polydextrose. GOS/inulin (9 : 1) and fructo-oligosaccharides (FOS)/inulin mixtures supported mostly similar growth abilities to those obtained for GOS and FOS, respectively. Microbial consumption of GOS, as determined by high-performance anion-exchange chromatography with pulsed amperometric detection, was evident for both lactobacilli and bifidobacteria.
These results may allow for the rational prediction of lactobacilli and/or bifidobacteria to be used in conjunction with prebiotics, such as GOS, as synbiotics.
评估特定碳水化合物（包括市售产品）支持两种知名肠道共生菌代表（即乳杆菌和双歧杆菌）生长的能力。
测试了 68 株细菌，代表 29 株人源乳杆菌和 39 株双歧杆菌（人源和动物源），以评估它们代谢 10 种不同碳水化合物的能力。使用终 OD600 、终 pH、发酵终产物和生长速率等诊断参数组合分析生长和代谢活性。
本研究中收集的数据提供了关于一系列碳水化合物促进生长特性的重要补充和比较信息，同时还研究了乳杆菌和/或双歧杆菌在碳水化合物利用能力方面的种间差异。半乳糖低聚糖（GOS）和乳果糖表现出最有利于生长的特性，而菊粉、麦芽糊精和聚右旋糖对乳杆菌和双歧杆菌的生长较差。GOS/菊粉（9:1）和果寡糖（FOS）/菊粉混合物支持的生长能力与 GOS 和 FOS 分别获得的生长能力相似。高效阴离子交换色谱-脉冲安培检测法证实了 GOS 被乳杆菌和双歧杆菌消耗。
这些结果可以允许合理预测乳杆菌和/或双歧杆菌与 GOS 等益生元结合使用，作为合生元。
