Raote Ishier, Saxena Sonashree, Campelo Felix, Malhotra Vivek
Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology, Barcelona 08003, Spain.
Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology, Barcelona 08003, Spain.
Biochim Biophys Acta Biomembr. 2021 Nov 1;1863(11):183700. doi: 10.1016/j.bbamem.2021.183700. Epub 2021 Jul 19.
TANGO1 protein facilitates the endoplasmic reticulum (ER) export of large cargoes that cannot be accommodated in 60 nm transport vesicles. It assembles into a ring in the plane of the ER membrane to create a distinct domain. Its lumenal portion collects and sorts folded cargoes while its cytoplasmic domains collar COPII coats, recruit retrograde COPI-coated membranes that fuse within the TANGO1 ring, thus opening a tunnel for cargo transfer from the ER into a growing export conduit. This mode of cargo transfer bypasses the need for vesicular intermediates and is used to export the most abundant and bulky cargoes. The evolution of TANGO1 and its activities defines the difference between yeast and animal early secretory pathways.
TANGO1蛋白促进了无法装入60纳米运输囊泡的大型货物从内质网（ER）输出。它在内质网膜平面上组装成一个环，形成一个独特的区域。其腔部收集并分类折叠好的货物，而其胞质结构域环绕COPII衣被，招募在内质网高尔基体中间隔室（ERGIC）中融合的逆行COPI包被膜，从而为货物从内质网转移到正在生长的输出管道开辟一条通道。这种货物转移模式无需囊泡中间体，并用于输出最丰富和最大体积的货物。TANGO1及其活性的进化定义了酵母和动物早期分泌途径之间的差异。
