Katiyar Priya, Pandey Neha, Varghese Boby, Sahu Keshav Kant
School of Studies in Biotechnology, Pt. Ravishankar Shukla University, Raipur 492 010, India.
Centre for Academic Success in Science and Engineering, University of KwaZulu-Natal, Durban 4001, South Africa.
Plants (Basel). 2025 Apr 16;14(8):1223. doi: 10.3390/plants14081223.
Plant growth-promoting bacteria (PGPB) are free-living microorganisms that actively reside in the rhizosphere and affect plant growth and development. These bacteria employ their own metabolic system to fix nitrogen, solubilize phosphate, and secrete hormones to directly impact the metabolism of plants. Generating sustainable agricultural production under various environmental stresses requires a detailed understanding of mechanisms that bacteria use to promote plant growth. In the present study, (MW843625), a PGP soil bacterium with a minimum inhibitory concentration (MIC) of 150 mM against fluoride (F), was isolated from agricultural fields of Chhattisgarh, India, and was assessed for remedial and PGP potential. This study concentrated on biomass accumulation, nutrient absorption, and oxidative stress tolerance in plants involving antioxidative enzymes. By determining MDA accumulation and ROS (O• and HO) in L. under F (50 ppm) stress, oxidative stress tolerance was assessed. The results showed that inoculation with enhanced the ability of L. seedlings to absorb nutrients and increased the amounts of total chlorophyll (Chl), total soluble protein, and biomass. In contrast to plants cultivated under F-stress alone, those inoculated with along with F showed considerably reduced concentrations of F in their roots, shoots, and grains. The alleviation of deleterious effects of F-stress on plants owing to inoculation has been associated with improved activity/upregulation of antioxidative genes (SOD, CAT, and APX) in comparison to only F-subjected plants, which resulted in lower O•, HO, and MDA content. Additionally, it has also been reflected from our study that has the potential to increase the activities of soil enzymes such as urease, phosphatase, dehydrogenase, nitrate reductase, and cellulase. Accordingly, the findings of the conducted study suggest that can be exploited not only as an ideal candidate for bioremediation but also for enhancing soil fertility and the promotion of growth and development of L. under F contamination.
植物促生细菌（PGPB）是一类自由生活的微生物，活跃于根际，影响植物的生长发育。这些细菌利用自身的代谢系统来固氮、溶解磷并分泌激素，从而直接影响植物的新陈代谢。在各种环境胁迫下实现可持续农业生产，需要详细了解细菌促进植物生长的机制。在本研究中，从印度恰蒂斯加尔邦的农田中分离出一株PGP土壤细菌（MW843625），其对氟（F）的最低抑菌浓度（MIC）为150 mM，并对其修复和PGP潜力进行了评估。本研究聚焦于植物中涉及抗氧化酶的生物量积累、养分吸收和氧化应激耐受性。通过测定在F（50 ppm）胁迫下番茄中丙二醛（MDA）的积累和活性氧（O•和HO），评估氧化应激耐受性。结果表明，接种该细菌增强了番茄幼苗吸收养分的能力，增加了总叶绿素（Chl）、总可溶性蛋白和生物量的含量。与仅在F胁迫下种植的植物相比，接种该细菌并同时施加F的植物，其根、茎和籽粒中的F浓度显著降低。与仅受F胁迫的植物相比，接种该细菌减轻了F胁迫对植物的有害影响，这与抗氧化基因（超氧化物歧化酶、过氧化氢酶和抗坏血酸过氧化物酶）活性提高/上调有关，从而导致较低的O•、HO和MDA含量。此外，我们的研究还表明，该细菌有潜力提高土壤酶如脲酶、磷酸酶、脱氢酶、硝酸还原酶和纤维素酶的活性。因此，本研究结果表明，该细菌不仅可作为生物修复的理想候选菌株，还可用于提高土壤肥力以及促进受F污染环境下番茄的生长发育。
