Teranishi K, Hamada K, Watanabe H
Mutat Res. 1978 Jan;56(3):273-80. doi: 10.1016/0027-5107(78)90193-8.
Air-borne particulate matter was collected on a filter, then extracted with benzene. The benzene-soluble material was separated into 5 fractions, namely acidic, basic, alipathic, polyaromatic and oxygenated fractions. The mutagenic activities of these fractions were examined with a set of Salmonella typhimurium mutants. The 6 mutants were from the TA1535 series, deep rough strains without excision repair, namely TA100 and TA98 (having a resistance-transfer factor) and the standard strain TA1535, TA1536, TA1537 and TA1538. Linear dose-response curves were obtained for the benzene-soluble organic matter, and its acidic, polyaromatic and oxygenated fractions with strain TA98 and a 9000 X g liver supernatant from both phenobarbital(PB)- and dibenz(a,h)anthracene(DBA)-treated rats. Among the 5 fractions tested, 3 fractions, namely the acidic, polyaromatic and oxygenated, played an important role in the mutagenicity of the benzene-soluble organic matter derived from air-borne particulate matter. The 9000 X g rat-liver supernatant was not required to make the acidic fraction mutagenic.
空气中的颗粒物收集在滤膜上，然后用苯萃取。苯溶性物质被分离成5个组分，即酸性组分、碱性组分、脂肪族组分、多环芳烃组分和含氧化合物组分。用一组鼠伤寒沙门氏菌突变体检测这些组分的诱变活性。这6个突变体来自TA1535系列，是无切除修复功能的深度粗糙菌株，即TA100和TA98（带有抗性转移因子）以及标准菌株TA1535、TA1536、TA1537和TA1538。对于苯溶性有机物及其酸性、多环芳烃和含氧化合物组分，在TA98菌株以及来自经苯巴比妥（PB）和二苯并(a,h)蒽（DBA）处理的大鼠的9000×g肝脏上清液存在的情况下，获得了线性剂量反应曲线。在所测试的5个组分中，酸性、多环芳烃和含氧化合物这3个组分在源自空气中颗粒物的苯溶性有机物的诱变性中起重要作用。使酸性组分具有诱变性不需要9000×g大鼠肝脏上清液。
