从京都市桂川水中回收的XAD-2醚提取物及其馏分在鼠伤寒沙门氏菌测试菌株中的致突变性。

Mutagenicity in Salmonella typhimurium tester strains of XAD-2-ether extract, recovered from Katsura River water in Kyoto City, and its fractions.

作者信息

Maruoka S, Yamanaka S

出版信息

Mutat Res. 1982 Jul-Aug;102(1):13-26. doi: 10.1016/0165-1218(82)90142-2.

Abstract

The mutagenic activity of XAD-2-ether extracts recovered from Katsura River water at monthly intervals during September to December 1980 was tested on S. typhimurium TA1538, TA1535, TA98 and TA100. The extracts showed strong mutagenic activity towards TA1538 nd TA98, especially in the presence of S9 mix. They were more active to TA1538 than to TA98. Some of each of the XAD-2-ether extracts were pooled and separated into neutral, basic and acidic fractions, and their mutagenic activities were tested on TA1538 and TA98 to determine their contribution to the total mutagenic activity of the parent extract. The neutral fraction was responsible for most of the total mutagenic activity of the parent extract. Although the basic fraction was only 5.4% by weight of the parent extract, it was much more mutagenic than any other fraction. Its contribution to the total mutagenic activity was higher than the acidic fraction which was 30.5% by weight of the parent extract.

摘要

1980年9月至12月期间，每月从桂川河水中回收的XAD - 2醚提取物的诱变活性，在鼠伤寒沙门氏菌TA1538、TA1535、TA98和TA100上进行了测试。提取物对TA1538和TA98显示出很强的诱变活性，尤其是在存在S9混合物的情况下。它们对TA1538的活性比对TA98更强。将每种XAD - 2醚提取物中的一部分合并，分离成中性、碱性和酸性馏分，并在TA1538和TA98上测试它们的诱变活性，以确定它们对母体提取物总诱变活性的贡献。中性馏分对母体提取物的总诱变活性起主要作用。虽然碱性馏分仅占母体提取物重量的5.4%，但其诱变作用比任何其他馏分都要强得多。它对总诱变活性的贡献高于酸性馏分，酸性馏分占母体提取物重量的30.5%。