Laboratoire de Virologie, Institut des Agents Infectieux, Laboratoire associé au Centre National de Référence des virus des infections respiratoires, Hospices Civils de Lyon, Lyon, France.
CIRI, Centre International de Recherche en Infectiologie, Team VirPath, Inserm, U1111, Université Claude Bernard Lyon 1, CNRS, UMR5308, ENS de Lyon, 69007, Lyon, France.
Sci Rep. 2021 Jul 22;11(1):14977. doi: 10.1038/s41598-021-93828-y.
A comprehensive clinical and microbiological assessments of COVID-19 in front-line healthcare workers (HCWs) is needed. Between April 10th and May 28th, 2020, 319 HCWs with acute illness were reviewed. In addition to SARS-CoV-2 RT-PCR screening, a multiplex molecular panel was used for testing other respiratory pathogens. For SARS-CoV-2 positive HCWs, the normalized viral load, viral culture, and virus neutralization assays were performed weekly. For SARS-CoV-2 negative HCWs, SARS-CoV-2 serological testing was performed one month after inclusion. Among the 319 HCWs included, 67 (21.0%) were tested positive for SARS-CoV-2; 65/67 (97.0%) developed mild form of COVID-19. Other respiratory pathogens were found in 6/66 (9.1%) SARS-CoV-2 positive and 47/241 (19.5%) SARS-Cov-2 negative HCWs (p = 0.07). The proportion of HCWs with a viral load > 5.0 log cp/mL (Ct value < 25) was less than 15% at 8 days after symptom onset; 12% of HCWs were positive after 40 days (Ct > 37). More than 90% of cultivable virus had a viral load > 4.5 log cp/mL (Ct < 26) and were collected within 10 days after symptom onset. Among negative HCWs, 6/190 (3.2%) seroconverted. Our data suggest that the determination of viral load can be used for appreciating the infectiousness of infected HCWs. These data could be helpful for facilitating their return to work.
需要对一线医护人员（HCWs）中的 COVID-19 进行全面的临床和微生物学评估。在 2020 年 4 月 10 日至 5 月 28 日期间，共回顾了 319 名患有急性疾病的 HCWs。除了 SARS-CoV-2 RT-PCR 筛查外，还使用多重分子面板检测其他呼吸道病原体。对于 SARS-CoV-2 阳性的 HCWs，每周进行标准化病毒载量、病毒培养和病毒中和测定。对于 SARS-CoV-2 阴性的 HCWs，在纳入后一个月进行 SARS-CoV-2 血清学检测。在纳入的 319 名 HCWs 中，有 67 名（21.0%）SARS-CoV-2 检测呈阳性；65/67（97.0%）患有 COVID-19 轻症。在 6/66（9.1%）SARS-CoV-2 阳性和 47/241（19.5%）SARS-CoV-2 阴性的 HCWs 中发现了其他呼吸道病原体（p=0.07）。症状出现后 8 天，病毒载量>5.0 log cp/mL（Ct 值<25）的 HCWs 比例不到 15%；12%的 HCWs 在 40 天后（Ct>37）呈阳性。>90%可培养的病毒病毒载量>4.5 log cp/mL（Ct<26），并在症状出现后 10 天内采集。在阴性 HCWs 中，有 6/190（3.2%）出现血清转换。我们的数据表明，病毒载量的测定可用于评估感染 HCWs 的传染性。这些数据有助于促进他们重返工作岗位。
