Erdoğan Eliuz Elif Ayşe
Technical Sciences Vocational School, Department of Food Technology, Mersin University, 33343 Mersin, Turkey.
Indian J Microbiol. 2021 Sep;61(3):291-297. doi: 10.1007/s12088-021-00939-1. Epub 2021 Apr 10.
In this study, the aerial parts of were subjected to hydrodistillation and the resulting compounds were analyzed in GC-MS. Antimicrobial activity of essential oil (EO) against , and were determined by agar well diffusion assay and microdilution method. Antimicrobial mechanism of the EO were investigated based on change of TTC-dehydrogenase relative, leakages of intracellular protein, DNA and potassium ion (K +). The main components of the EO were β-pinene, 1,8-cineole, camphor, α-pinene, 4-terpineol, and 4-thujanol. The MICs of the EO against the microorganisms were 15.2 mg/mL for 17.5 mg/mL for and 28.8 mg/mL for The inhibition zones were 18.16 mm, 25.01 mm, and 27.01 mm for , , and respectively ( < 0.05). An increase in DNA, protein and K leakage was observed when microorganisms were exposed to the EO. The TTC-DRA of the treated microorganism cells was also significantly decreased because of slowing the respiration. The present study provided an experimental basis of practical application of EO as a natural agent.
The online version contains supplementary material available at 10.1007/s12088-021-00939-1.
在本研究中，对[植物名称]的地上部分进行水蒸馏，并通过气相色谱 - 质谱联用仪（GC - MS）分析所得化合物。采用琼脂孔扩散法和微量稀释法测定[植物名称]精油（EO）对[微生物名称1]、[微生物名称2]和[微生物名称3]的抗菌活性。基于氯化三苯基四氮唑（TTC） - 脱氢酶相对变化、细胞内蛋白质、DNA和钾离子（K⁺）泄漏情况研究了该精油的抗菌机制。该精油的主要成分是β - 蒎烯、1,8 - 桉叶素、樟脑、α - 蒎烯、4 - 松油醇和4 - 侧柏醇。该精油对这些微生物的最低抑菌浓度（MIC）分别为：对[微生物名称1]为15.2毫克/毫升，对[微生物名称2]为17.5毫克/毫升，对[微生物名称3]为28.8毫克/毫升。对[微生物名称1]、[微生物名称2]和[微生物名称3]的抑菌圈分别为18.16毫米、25.01毫米和27.01毫米（P < 0.05）。当微生物暴露于该精油时，观察到DNA、蛋白质和K⁺泄漏增加。由于呼吸减慢，处理过的微生物细胞的TTC - 脱氢酶活性也显著降低。本研究为[植物名称]精油作为天然制剂的实际应用提供了实验依据。
在线版本包含可在10.1007/s12088 - 021 - 00939 - 1获取的补充材料。
