Graduate Institute of Sports Science, Natioal Taiwan Sport University, Taoyuan City 33301, Taiwan.
Food Funct. 2021 Sep 7;12(17):7728-7740. doi: 10.1039/d0fo02939d. Epub 2021 Jul 23.
Type 2 diabetes mellitus (T2DM) is a prevalent chronic disease characterized by hyperglycemia and insulin resistance. Regular exercise is one of the effective lifestyle interventions for maintaining healthy weight and blood glucose levels in the normal range and lowering risk factors. Probiotics, live microorganisms that are beneficial to health, are involved in the regulation of host metabolism. We thus hypothesize that the combination of exercise training and Bifidobacterium longum OLP-01 (OLP-01) could improve insulin sensitivity, blood glucose control and body composition in db/db mice. Twenty-four C57BL/6 J db/db male mice (20-weeks old) were divided into four groups (n = 6 per group): vehicle, OLP-01 supplementation (OLP-01), exercise training (EX) and exercise training with OLP-01 supplementation (EX + OLP-01). Animals in the EX and EX + OLP-01 groups underwent strength exercise training for 6 weeks, 5 days per week. After the exercise training, we tested forelimb grip strength, exhaustive running, oral glucose tolerance test (OGTT) and serum biomarkers. Results: Combined intervention of EX and OLP-01 prevented elevation of body weight and body fat. Grip strength and exhaustive swimming time were significantly higher in the EX + OLP-01 group than in the other groups. We found that EX OLP-01 reduced glycolipid parameters (fasting blood glucose and hemoglobin A1c), improved insulin sensitivity (oral glucose tolerance test and HOMA-IR), relieved liver injury parameters (aspartate aminotransferase and alanine aminotransferase) and repaired pancreas damage. Based on our findings, we speculate that the positive effects of combining EX with OLP-01 on capacity for physical activity, blood glucose control and body composition suggest an integrative approach to treating type 2 diabetes. Altogether, the combination of EX with OLP-01 treatment might be a good candidate for preventing and treating diabetes.
2 型糖尿病（T2DM）是一种常见的慢性疾病，其特征是高血糖和胰岛素抵抗。定期运动是维持健康体重和血糖水平在正常范围内并降低风险因素的有效生活方式干预措施之一。益生菌是对健康有益的活体微生物，参与宿主代谢的调节。因此，我们假设运动训练和长双歧杆菌 OLP-01（OLP-01）的联合应用可以改善 db/db 小鼠的胰岛素敏感性、血糖控制和身体成分。将 24 只 20 周龄的 C57BL/6 J db/db 雄性小鼠分为四组（每组 6 只）：对照组、OLP-01 补充组（OLP-01）、运动训练组（EX）和运动训练与 OLP-01 补充组（EX+OLP-01）。EX 和 EX+OLP-01 组的动物进行了 6 周的力量运动训练，每周 5 天。运动训练结束后，我们测试了前肢握力、衰竭游泳、口服葡萄糖耐量试验（OGTT）和血清生物标志物。结果：EX 和 OLP-01 的联合干预防止了体重和体脂肪的增加。EX+OLP-01 组的握力和衰竭游泳时间明显高于其他组。我们发现，EX+OLP-01 降低了糖脂参数（空腹血糖和糖化血红蛋白），改善了胰岛素敏感性（口服葡萄糖耐量试验和 HOMA-IR），缓解了肝损伤参数（天冬氨酸转氨酶和丙氨酸转氨酶），修复了胰腺损伤。基于我们的发现，我们推测将 EX 与 OLP-01 联合应用对体力活动能力、血糖控制和身体成分的积极影响表明了一种综合治疗 2 型糖尿病的方法。总的来说，EX 与 OLP-01 联合治疗可能是预防和治疗糖尿病的一个很好的候选方案。
