Forensic DNA Division, National Forensic Service, Wonju, Korea.
J Forensic Sci. 2021 Nov;66(6):2362-2368. doi: 10.1111/1556-4029.14812. Epub 2021 Jul 23.
Dogs (Canis lupus familiaris) are among the most common companion animals in the Republic of Korea. Recently, there have been many criminal cases of dog cruelty, injury, and theft, among others. This has increased the importance of dog-related biological evidence at crime scenes. The National Forensic Service of the Republic of Korea conducts short tandem repeat (STR) analysis using the Thermo Scientific Canine Genotypes™ Panel 2.1 Kit (Canine Kit) to identify individual dogs through forensic analysis. The Canine Kit was developed as a forensic STR kit for the identification of individual dogs. However, an allelic ladder was neither developed nor included in the commercial kit, leaving an issue of accurate genotyping. Primer details for the 18 markers used in the Canine Kit are proprietary information, and thus, unavailable to end-users. In this study, an allelic ladder was constructed with 160 fragments by combining 158 fragments of STR alleles obtained by nested PCR and two fragments artificially obtained from the sex-determination marker. By including the new allelic ladder in analysis of samples amplified with the Canine Kit, the accuracy and reliability of data analysis were improved. Application of this allelic ladder would be helpful for interlaboratory data sharing and standardization of canine genotype databases.
狗（Canis lupus familiaris）是大韩民国最常见的伴侣动物之一。最近，发生了许多虐待狗、伤害狗和盗窃狗等与狗有关的犯罪案件。这增加了犯罪现场与狗有关的生物证据的重要性。大韩民国国家法医服务局使用赛默飞世尔科技犬种基因型™面板 2.1 试剂盒（犬试剂盒）进行短串联重复（STR）分析，通过法医分析来识别个体狗。犬试剂盒是作为一种法医 STR 试剂盒开发的，用于识别个体狗。然而，商业试剂盒中既没有开发也没有包含等位基因梯，这导致了准确基因分型的问题。犬试剂盒中使用的 18 个标记物的引物详细信息是专有的，因此无法提供给最终用户。在这项研究中，通过组合嵌套 PCR 获得的 158 个 STR 等位基因片段和从性别决定标记人工获得的两个片段，构建了一个包含 160 个片段的等位基因梯。通过在犬试剂盒扩增的样本分析中包含新的等位基因梯，提高了数据分析的准确性和可靠性。应用该等位基因梯有助于实验室间数据共享和犬基因型数据库的标准化。
