Department of Small Animal Medicine and Surgery, College of Veterinary Medicine, University of Georgia, Athens, GA 30602.
Department of Population Health, College of Veterinary Medicine, University of Georgia, Athens, GA 30602.
Am J Vet Res. 2021 Aug;82(8):659-666. doi: 10.2460/ajvr.82.8.659.
To identify differential microRNA (miRNA) expression in dogs with splenic hemangiosarcoma, splenic hematoma, and histologically normal spleens.
Dogs with splenic hemangiosarcoma (n = 10), splenic hematoma (n = 5), and histologically normal spleens (n = 5).
Splenic tissue and serum samples were collected from dogs with splenic masses (ie, hemangiosarcoma or hematoma samples) and healthy control dogs (ie, control samples), and total RNA was extracted. Reverse transcription quantitative real-time PCR was performed with 28 miRNAs associated with hemangiosarcoma, angiosarcoma, or associated genes. Differential expression analysis was performed.
Control tissue and serum samples had similar miRNA expression patterns, and hemangiosarcoma tissue and serum samples did not. Hemangiosarcoma serum samples had higher expression than hemangiosarcoma tissue for 13 miRNAs and lower expression for 1 miRNA. Control tissue and hemangiosarcoma tissue had varying expressions for 12 miRNAs, with 10 more highly expressed in control samples and 2 more highly expressed in hemangiosarcoma samples. Five miRNAs (miR-214-3p, miR-452, miR-494-3p, miR-497-5p, miR-543) had significantly different expression in serum between dogs with splenic masses (ie, hemangiosarcoma or hematoma) and serum of dogs with histologically normal spleens, with higher expression in the serum of dogs with splenic masses for all 5 miRNAs.
5 circulating miRNAs were identified that distinguished dogs with splenic hemangiosarcoma or hematoma from those with histologically normal spleens. These 5 miRNAs had higher expression in dogs with splenic masses, indicating upregulation of these circulating miRNAs occurs in these splenic disease states. These miRNAs may be useful as a noninvasive screening tool that uses serum to identify dogs with splenic masses.
鉴定患有脾血管肉瘤、脾血肿和组织学正常脾脏的狗之间差异表达的 microRNA（miRNA）。
患有脾血管肉瘤（n=10）、脾血肿（n=5）和组织学正常脾脏的狗（n=5）。
从患有脾肿块（即血管肉瘤或血肿样本）和健康对照狗（即对照样本）的狗中采集脾组织和血清样本，并提取总 RNA。进行与血管肉瘤、血管肉瘤或相关基因相关的 28 个 miRNA 的逆转录定量实时 PCR。进行差异表达分析。
对照组织和血清样本具有相似的 miRNA 表达模式，而血管肉瘤组织和血清样本则没有。血管肉瘤血清样本中 13 个 miRNA 的表达高于血管肉瘤组织，1 个 miRNA 的表达低于血管肉瘤组织。对照组织和血管肉瘤组织对 12 个 miRNA 的表达存在差异，其中 10 个在对照样本中表达更高，2 个在血管肉瘤样本中表达更高。在脾肿块（即血管肉瘤或血肿）和组织学正常脾脏的狗之间，血清中有 5 个 miRNA（miR-214-3p、miR-452、miR-494-3p、miR-497-5p、miR-543）的表达存在显著差异，所有 5 个 miRNA 在脾肿块狗的血清中的表达均更高。
鉴定出 5 种循环 miRNA，可区分患有脾血管肉瘤或血肿的狗与组织学正常脾脏的狗。这 5 个 miRNA 在脾肿块狗中的表达更高，表明这些循环 miRNA 在这些脾脏疾病状态中上调。这些 miRNA 可能作为一种非侵入性的筛选工具，使用血清识别患有脾肿块的狗。
