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基因型对高硫苷合成品系 的影响。

Influence of Genotype on High Glucosinolate Synthesis Lines of .

机构信息

Genomics Division, Department of Agricultural Bio-Resources, National Institute of Agricultural Sciences, Rural Development Administration, Wansan-gu, Jeonju 54874, Korea.

Bioinformatics Team of Theragen Etex Institute, Suwon 16229, Korea.

出版信息

Int J Mol Sci. 2021 Jul 7;22(14):7301. doi: 10.3390/ijms22147301.

DOI:10.3390/ijms22147301
PMID:34298919
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8305852/
Abstract

This study was conducted to investigate doubled haploid (DH) lines produced between high GSL (HGSL) ssp. (yellow sarson) and low GSL (LGSL) ssp. (pak choi) parents. In total, 161 DH lines were generated. GSL content of HGSL DH lines ranged from 44.12 to 57.04 μmol·g·dry weight (dw), which is within the level of high GSL ssp. (47.46 to 59.56 μmol g dw). We resequenced five of the HGSL DH lines and three of the LGSL DH lines. Recombination blocks were formed between the parental and DH lines with 108,328 single-nucleotide polymorphisms in all chromosomes. In the measured GSL, gluconapin occurred as the major substrate in HGSL DH lines. Among the HGSL DH lines, BrYSP_DH005 had glucoraphanin levels approximately 12-fold higher than those of the HGSL mother plant. The hydrolysis capacity of GSL was analyzed in HGSL DH lines with a Korean pak choi cultivar as a control. Bioactive compounds, such as 3-butenyl isothiocyanate, 4-pentenyl isothiocyanate, 2-phenethyl isothiocyanate, and sulforaphane, were present in the HGSL DH lines at 3-fold to 6.3-fold higher levels compared to the commercial cultivar. The selected HGSL DH lines, resequencing data, and SNP identification were utilized for genome-assisted selection to develop elite GSL-enriched cultivars and the industrial production of potential anti-cancerous metabolites such as gluconapin and glucoraphanin.

摘要

本研究旨在调查高硫代葡萄糖苷 (GSL) 亚种 (黄芥菜) 和低硫代葡萄糖苷 (LGSL) 亚种 (白菜) 亲本之间产生的双单倍体 (DH) 系。共生成 161 个 DH 系。HGSL DH 系的 GSL 含量在 44.12 到 57.04 μmol·g·干重 (dw) 之间，处于高 GSL 亚种的水平 (47.46 到 59.56 μmol g dw)。我们对 5 个 HGSL DH 系和 3 个 LGSL DH 系进行了重测序。在所有染色体中，亲本和 DH 系之间形成了 108328 个单核苷酸多态性的重组块。在所测量的 GSL 中，HGSL DH 系中的主要底物是葡萄糖芥苷。在 HGSL DH 系中，BrYSP_DH005 的萝卜硫苷含量比 HGSL 母株高约 12 倍。以韩国白菜品种为对照，分析了 HGSL DH 系的 GSL 水解能力。生物活性化合物，如 3-丁烯基异硫氰酸酯、4-戊烯基异硫氰酸酯、2-苯乙基异硫氰酸酯和萝卜硫素，在 HGSL DH 系中的含量比商业品种高 3 倍至 6.3 倍。所选 HGSL DH 系、重测序数据和 SNP 鉴定被用于基因组辅助选择，以开发富含 GSL 的优良品种，并进行潜在抗癌代谢物如葡萄糖芥苷和萝卜硫素的工业生产。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64c9/8305852/910954920f78/ijms-22-07301-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64c9/8305852/e5f5ccb5adc6/ijms-22-07301-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64c9/8305852/efeda24bed18/ijms-22-07301-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64c9/8305852/7d6ca2e3eed5/ijms-22-07301-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64c9/8305852/7acefa489470/ijms-22-07301-g004a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64c9/8305852/910954920f78/ijms-22-07301-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64c9/8305852/e5f5ccb5adc6/ijms-22-07301-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64c9/8305852/efeda24bed18/ijms-22-07301-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64c9/8305852/7d6ca2e3eed5/ijms-22-07301-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64c9/8305852/7acefa489470/ijms-22-07301-g004a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64c9/8305852/910954920f78/ijms-22-07301-g006.jpg

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