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蔬菜型和油籽型植物中控制硫代葡萄糖苷含量的主要QTL基因的转录组和QTL定位分析。

Transcriptome and QTL mapping analyses of major QTL genes controlling glucosinolate contents in vegetable- and oilseed-type plants.

作者信息

Kim Jin A, Moon Heewon, Kim Hyang Suk, Choi Dasom, Kim Nan-Sun, Jang Juna, Lee Sang Woo, Baskoro Dwi Nugroho Adji, Kim Dong-Hwan

机构信息

Department of Agricultural Biotechnology, National Institute of Agricultural Science, Rural Development Administration, Jeonju, Jeollabuk-do, Republic of Korea.

Department of Plant Science and Technology, Chung-Ang University, Anseong,  Republic of Korea.

出版信息

Front Plant Sci. 2023 Jan 18;13:1067508. doi: 10.3389/fpls.2022.1067508. eCollection 2022.

DOI:10.3389/fpls.2022.1067508
PMID:36743533
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9891538/
Abstract

Glucosinolates (GSLs) are secondary metabolites providing defense against pathogens and herbivores in plants, and anti-carcinogenic activity against human cancer cells. Profiles of GSLs vary greatly among members of genus . In this study, we found that a reference line of Chinese cabbage ( ssp. ), 'Chiifu' contains significantly lower amounts of total GSLs than the oilseed-type ( ssp. ) line 'LP08'. This study aimed to identify the key regulators of the high accumulation of GSLs in plants using transcriptomic and linkage mapping approaches. Comparative transcriptome analysis showed that, in total, 8,276 and 9,878 genes were differentially expressed between 'Chiifu' and 'LP08' under light and dark conditions, respectively. Among 162 GSL pathway genes, 79 were related to GSL metabolism under light conditions. We also performed QTL analysis using a single nucleotide polymorphism-based linkage map constructed using 151 F individuals derived from a cross between the 'Chiifu' and 'LP08' inbred lines. Two major QTL peaks were successfully identified on chromosome 3 using high-performance liquid chromatography to obtain GSL profiles from 97 F recombinant inbred lines. The MYB-domain transcription factor gene (Bra012961) was found in the highest QTL peak region. The second highest peak was located near the 2-oxoacid-dependent dioxygenase gene (Bra022920). This study identified major genes responsible for differing profiles of GSLs between 'Chiifu' and 'LP08'. Thus, our study provides molecular insights into differences in GSL profiles between vegetative- and oilseed-type plants.

摘要

硫代葡萄糖苷（GSLs）是植物中的次生代谢产物，可抵御病原体和食草动物，并对人类癌细胞具有抗癌活性。GSLs的谱在大白菜属成员间差异很大。在本研究中，我们发现大白菜（亚种）的一个参考品系‘Chiifu’的总GSLs含量明显低于油籽型（亚种）品系‘LP08’。本研究旨在利用转录组学和连锁图谱方法鉴定大白菜中GSLs高积累的关键调控因子。比较转录组分析表明，在光照和黑暗条件下，‘Chiifu’和‘LP08’之间分别共有8276个和9878个基因差异表达。在162个GSL途径基因中，79个与光照条件下的GSL代谢相关。我们还使用基于单核苷酸多态性的连锁图谱进行了QTL分析，该图谱是利用来自‘Chiifu’和‘LP08’自交系杂交产生的151个F个体构建的。使用高效液相色谱法从97个F重组自交系中获得GSL谱，在第3号染色体上成功鉴定出两个主要的QTL峰。在最高的QTL峰区域发现了MYB结构域转录因子基因（Bra012961）。第二高的峰位于2-氧代酸依赖性双加氧酶基因（Bra022920）附近。本研究鉴定了导致‘Chiifu’和‘LP08’之间GSLs谱不同的主要基因。因此，我们的研究为营养型和油籽型大白菜之间GSL谱的差异提供了分子见解。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddfb/9891538/e67f159dddfc/fpls-13-1067508-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddfb/9891538/4bd3633da579/fpls-13-1067508-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddfb/9891538/8ed6b342c1bc/fpls-13-1067508-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddfb/9891538/06ed67fe5b40/fpls-13-1067508-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddfb/9891538/a918f4af3429/fpls-13-1067508-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddfb/9891538/9544a8549319/fpls-13-1067508-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddfb/9891538/fbc02e778265/fpls-13-1067508-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddfb/9891538/0b09c39f5f88/fpls-13-1067508-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddfb/9891538/713f9365cc47/fpls-13-1067508-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddfb/9891538/e67f159dddfc/fpls-13-1067508-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddfb/9891538/4bd3633da579/fpls-13-1067508-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddfb/9891538/8ed6b342c1bc/fpls-13-1067508-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddfb/9891538/06ed67fe5b40/fpls-13-1067508-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddfb/9891538/a918f4af3429/fpls-13-1067508-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddfb/9891538/9544a8549319/fpls-13-1067508-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddfb/9891538/fbc02e778265/fpls-13-1067508-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddfb/9891538/0b09c39f5f88/fpls-13-1067508-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddfb/9891538/713f9365cc47/fpls-13-1067508-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddfb/9891538/e67f159dddfc/fpls-13-1067508-g009.jpg

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