Division of Cell Biology, Histology and Embryology, Gottfried Schatz Research Center, Medical University of Graz, 8010 Graz, Austria.
Int J Mol Sci. 2021 Jul 12;22(14):7464. doi: 10.3390/ijms22147464.
During pregnancy, freely floating placental villi are adapted to fluid shear stress due to placental perfusion with maternal plasma and blood. In vitro culture of placental villous explants is widely performed under static conditions, hoping the conditions may represent the in utero environment. However, static placental villous explant culture dramatically differs from the in vivo situation. Thus, we established a flow culture system for placental villous explants and compared commonly used static cultured tissue to flow cultured tissue using transmission and scanning electron microscopy, immunohistochemistry, and lactate dehydrogenase (LDH) and human chorionic gonadotropin (hCG) measurements. The data revealed a better structural and biochemical integrity of flow cultured tissue compared to static cultured tissue. Thus, this new flow system can be used to simulate the blood flow from the mother to the placenta and back in the most native-like in vitro system so far and thus can enable novel study designs.
在妊娠期间，由于胎盘灌注母体血浆和血液，游离漂浮的胎盘绒毛适应流体切应力。胎盘绒毛外植体的体外培养广泛在静态条件下进行，希望这些条件可以代表宫内环境。然而，静态胎盘绒毛外植体培养与体内情况有很大的不同。因此，我们建立了一个用于胎盘绒毛外植体的流动培养系统，并使用透射和扫描电子显微镜、免疫组织化学以及乳酸脱氢酶（LDH）和人绒毛膜促性腺激素（hCG）测量，将常用的静态培养组织与流动培养组织进行了比较。数据显示，与静态培养组织相比，流动培养组织具有更好的结构和生化完整性。因此，这种新的流动系统可以在迄今为止最接近天然的体外系统中模拟从母亲到胎盘再回到母亲的血流，从而可以实现新的研究设计。
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