Department of Landscape Architecture, School of Design, Shanghai Jiao Tong University, 800 Dong Chuan Road, Shanghai 200240, China.
R&D Center for Aromatic Plants, Shanghai Jiao Tong University, 800 Dong Chuan Road, Shanghai 200240, China.
Molecules. 2021 Jul 9;26(14):4171. doi: 10.3390/molecules26144171.
Tobacco ( Linn.) is a famous traditional herb used in folk medicine. The essential oils of tobacco have been demonstrated in modern studies to possess antioxidant, anti-inflammatory, and neuroprotective properties, while its anxiolytic effect has not been reported. The purpose of this study was to evaluate the anxiolytic effect of Yunnan tobacco essential oil (YTO) and Zimbabwe tobacco essential oil (ZTO) on mice. The constituents of YTO and ZTO were analyzed by GC/MS. The anxiolytic effect of YTO and ZTO (0.1%, 1%, and 10%, /) on male ICR mice was evaluated in the light-dark box test (LDB) and the elevated plus maze test (EPM) test via inhalation and transdermal administration. After the behavioral tests, salivary corticosterone levels in mice were measured. The behavioral analysis showed that the administration of both YTO and ZTO elevated the time that the mice spent in the light chamber in the LDB test compared to the untreated control. In the EPM test, YTO and ZTO increased the time spent in open arms and the number of entries into the open arms. In addition, both YTO and ZTO significantly decreased salivary corticosterone levels in mice ( ≤ 0.001). In summary, our results demonstrated that inhalation and transdermal administration of both YTO and ZTO showed anxiolytic effect on male ICR mice.
烟草（ Linn.）是民间医学中一种著名的传统草药。现代研究表明，烟草精油具有抗氧化、抗炎和神经保护作用，但其抗焦虑作用尚未报道。本研究旨在评价云南烟草精油（YTO）和津巴布韦烟草精油（ZTO）对小鼠的抗焦虑作用。采用 GC/MS 分析 YTO 和 ZTO 的成分。通过吸入和透皮给药，在明暗箱试验（LDB）和高架十字迷宫试验（EPM）中评价 YTO 和 ZTO（0.1%、1%和 10%，/）对雄性 ICR 小鼠的抗焦虑作用。行为测试后，测量小鼠唾液皮质酮水平。行为分析表明，与未处理的对照组相比，YTO 和 ZTO 给药均增加了小鼠在 LDB 试验中处于亮室的时间。在 EPM 试验中，YTO 和 ZTO 增加了进入开放臂的时间和次数。此外，YTO 和 ZTO 均显著降低了小鼠的唾液皮质酮水平（≤0.001）。总之，我们的结果表明，YTO 和 ZTO 的吸入和透皮给药对雄性 ICR 小鼠均表现出抗焦虑作用。
