Department of Biotechnology, School of Life Sciences, Pondicherry University, Puducherry, 605014, India.
Inflammation. 2020 Jun;43(3):937-950. doi: 10.1007/s10753-020-01179-z.
This study investigates the mode of action of Neophytadiene (MT), a molecule isolated from a marine algae Turbinaria ornata in LPS-induced inflammation in both in vitro and in vivo conditions. Neophytadiene (25, 50, 100 μM/mL) was treated to LPS-stimulated RAW 264.7 macrophages cells to identify its anti-inflammatory potential by measuring the level of tumour necrosis factor (TNF-α) by enzyme-linked immunosorbent assay (ELISA) and nitric oxide (NO) using Griess reagent. The mRNA levels of inflammatory cytokines, interleukin (IL-6 and IL-10), and the protein expression of nuclear factor-κB (NF-κB) and inducible nitric oxide synthase (iNOS) were quantified by Western blot analysis. Subsequently, Neophytadiene (12, 25, 50 mg/kg b.wt/p.o) was pre-treated for 7 days to the experimental animals followed by LPS (10 mg/kg) injection interaperitonially. After LPS induction, blood was collected and the haematological parameters were analysed followed by isolation of heart tissue for biochemical molecular and histopathological analysis Neophytadiene significantly inhibited the NO production and inflammatory cytokines TNF-α, IL-6 and IL-10 both in in vitro and in vivo conditions. Further, the expression of TNF-α, IL1β, NF-κB, iNOS, PI3k/Akt and MAPK in the heart tissue was modulated by Neophytadiene significantly confirming the anti-inflammatory potential. Thus, the effect of Neophytadiene on LPS-induced cardiac injury can be attributed to its anti-inflammatory antioxidant and cardioprotective properties.
本研究探讨了 Neophytadiene（MT）的作用模式，MT 是从海洋藻类 Turbinaria ornata 中分离得到的一种分子，在 LPS 诱导的炎症中具有体内和体外的抗炎作用。Neophytadiene（25、50、100μM/mL）处理 LPS 刺激的 RAW 264.7 巨噬细胞，通过酶联免疫吸附试验（ELISA）测量肿瘤坏死因子（TNF-α）的水平，并用 Griess 试剂测量一氧化氮（NO）的水平，以确定其抗炎潜力。通过 Western blot 分析定量炎性细胞因子白细胞介素（IL-6 和 IL-10）的 mRNA 水平以及核因子-κB（NF-κB）和诱导型一氧化氮合酶（iNOS）的蛋白表达。随后，Neophytadiene（12、25、50mg/kg b.wt/p.o）预处理实验动物 7 天，然后腹膜内注射 LPS（10mg/kg）。在 LPS 诱导后，采集血液并分析血液学参数，然后分离心脏组织进行生化分子和组织病理学分析。Neophytadiene 显著抑制了体外和体内条件下 NO 的产生和炎症细胞因子 TNF-α、IL-6 和 IL-10。此外，Neophytadiene 显著调节了心脏组织中 TNF-α、IL1β、NF-κB、iNOS、PI3k/Akt 和 MAPK 的表达，证实了其抗炎潜力。因此，Neophytadiene 对 LPS 诱导的心脏损伤的作用可归因于其抗炎、抗氧化和心脏保护特性。
