Chen Jishun, Ran Fengying, Chen Qinhua, Luo Dan, Ma Weidong, Han Tuo, Wang Ceming, Wang Congxia
Department of Cardiology, The Second Affiliated Hospital of Xi'an Jiaotong University Xi'an Shanxi 710004 China
Affiliated Dongfeng Hospital, Hubei University of Medicine Shiyan Hubei 442008 China.
RSC Adv. 2019 Feb 4;9(8):4463-4468. doi: 10.1039/c8ra09459d. eCollection 2019 Jan 30.
A sensitive biosensor using carbon dots and deoxyribonuclease I-aided target recycling signal amplification has been developed to detect myoglobin (MB), which is an important cardiac biomarker and plays a major role in the diagnosis of acute myocardial infarction (AMI). Here, in the absence of MB, the MB aptamer (Ap) is absorbed on the surface of carbon dots (CDs) through π-π stacking interactions, resulting in quenching of the fluorescent label by forming CD-aptamer complexes. Upon adding MB, the Ap sequences could be specifically recognized by MB, leading to the recovery of quenched fluorescence. Thus, quantitative evaluation of MB concentration has been achieved in a broad range from 50 pg mL to 100 ng mL, and the detection limit is as low as 20 pg mL. This strategy is capable of specific and sensitive detection of MB in human serum, urine, and saliva and can be used for the diagnosis of AMI in the future.
一种利用碳点和脱氧核糖核酸酶I辅助靶标循环信号放大的灵敏生物传感器已被开发用于检测肌红蛋白（MB），肌红蛋白是一种重要的心脏生物标志物，在急性心肌梗死（AMI）的诊断中起主要作用。在此，在不存在MB的情况下，MB适配体（Ap）通过π-π堆积相互作用吸附在碳点（CDs）表面，通过形成CD-适配体复合物导致荧光标记物淬灭。加入MB后，Ap序列可被MB特异性识别，从而使淬灭的荧光恢复。因此，在50 pg/mL至100 ng/mL的宽范围内实现了对MB浓度的定量评估，检测限低至20 pg/mL。该策略能够特异性且灵敏地检测人血清、尿液和唾液中的MB，未来可用于AMI的诊断。
