Department of Chemistry, Faculty of Science and Arts, Najran University, Najran 11001, Saudi Arabia.
Promising Centre for Sensors and Electronic Devices (PCSED), Najran University, Najran 11001, Saudi Arabia.
Biosensors (Basel). 2022 Dec 8;12(12):1151. doi: 10.3390/bios12121151.
In this study, using pure and copper-doped titanium dioxide (Cu-TiO) nanostructures as the base matrix, enzyme-less label free myoglobin detection to identify acute myocardial infarction was performed and presented. The Cu-TiO nanomaterials were prepared using facile sol-gel method. In order to comprehend the morphologies, compositions, structural, optical, and electrochemical characteristics, the pure and Cu-TiO nanomaterials were investigated by several techniques which clearly revealed good crystallinity and high purity. To fabricate the enzyme-less label free biosensor, thick films of synthesized nanomaterials were applied to the surface of a pre-fabricated gold screen-printed electrode (Au-SPE), which serves as a working electrode to construct the myoglobin (Mb) biosensors. The interference study of the fabricated biosensor was also carried out with human serum albumin (HSA) and cytochrome c (cyt-c). Interestingly, the Cu-doped TiO nanomaterial-based Mb biosensor displayed a higher sensitivity of 61.51 µAcm/nM and a lower detection limit of 14 pM with a response time of less than 10 ms.
在这项研究中，使用纯二氧化钛（TiO）和铜掺杂二氧化钛（Cu-TiO）纳米结构作为基质，进行了无酶标签的肌红蛋白检测，以用于识别急性心肌梗死，并进行了展示。Cu-TiO 纳米材料是通过简便的溶胶-凝胶法制备的。为了了解纯和 Cu-TiO 纳米材料的形貌、组成、结构、光学和电化学特性，使用了多种技术对其进行了研究，这些技术清楚地显示出了良好的结晶度和高纯度。为了制备无酶标签的生物传感器，将合成的纳米材料的厚膜应用于预制的金丝网印刷电极（Au-SPE）的表面，该电极作为工作电极来构建肌红蛋白（Mb）生物传感器。还对所制备的生物传感器进行了与人血清白蛋白（HSA）和细胞色素 c（cyt-c）的干扰研究。有趣的是，基于 Cu 掺杂 TiO 纳米材料的 Mb 生物传感器显示出更高的灵敏度为 61.51 µAcm/nM，更低的检测限为 14 pM，响应时间小于 10 ms。
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