VDF FutureCeuticals, Inc., Momence, IL 60954, USA.
University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
Molecules. 2021 Jul 16;26(14):4306. doi: 10.3390/molecules26144306.
Coffee cherry is a rich source of chlorogenic acids (CGAs) and caffeine. In this study we examined the potential antioxidant activity and enzyme inhibitory effects of whole coffee cherries (WCC) and their two extracts on α-amylase, α-glucosidase and acetylcholinesterase (AChE) activities, which are targets for the control of diabetes and Alzheimer's diseases. Whole coffee cherry extract 40% (WCCE1) is rich in chlorogenic acid compounds, consisting of a minimum of 40% major isomers, namely 3-caffeoylquinic acids, 4-caffeoylquinic acids, 5-caffeoylquinic acids, 3,4-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid, 4,5-dicaffeoylquinic acid, 4-feruloylquinc acid, and 5-feruloylquinc acid. Whole coffee cherry extract 70% (WCCE2) is rich in caffeine, with a minimum of 70%. WCCE1 inhibited the activities of digestive enzymes α-amylase and α-glucosidase, and WCCE2 inhibited acetylcholinesterase activities with their IC values of 1.74, 2.42, and 0.09 mg/mL, respectively. Multiple antioxidant assays-including DPPH, ABTS, FRAP, ORAC, HORAC, NORAC, and SORAC-demonstrated that WCCE1 has strong antioxidant activity.
咖啡樱桃是绿原酸（CGAs）和咖啡因的丰富来源。在这项研究中，我们研究了全咖啡樱桃（WCC）及其两种提取物对α-淀粉酶、α-葡萄糖苷酶和乙酰胆碱酯酶（AChE）活性的潜在抗氧化和酶抑制作用，这些靶点是控制糖尿病和阿尔茨海默病的关键。40%的全咖啡樱桃提取物（WCCE1）富含绿原酸化合物，至少由 40%的主要异构体组成，即 3-咖啡酰奎宁酸、4-咖啡酰奎宁酸、5-咖啡酰奎宁酸、3,4-二咖啡酰奎宁酸、3,5-二咖啡酰奎宁酸、4,5-二咖啡酰奎宁酸、4-阿魏酰奎宁酸和 5-阿魏酰奎宁酸。70%的全咖啡樱桃提取物（WCCE2）富含咖啡因，最低含量为 70%。WCCE1 抑制了消化酶α-淀粉酶和α-葡萄糖苷酶的活性，而 WCCE2 抑制了乙酰胆碱酯酶的活性，其 IC50 值分别为 1.74、2.42 和 0.09mg/mL。多种抗氧化测定方法，包括 DPPH、ABTS、FRAP、ORAC、HORAC、NORAC 和 SORAC，表明 WCCE1 具有很强的抗氧化活性。
