Marins Geani de Oliveira, Pires Thiago de Oliveira, Souza-Santos Reinaldo, Sobral Andréa, França Rafael Freitas de Oliveira, Azevedo Elisa de Almeida Neves, Sena Marília de Albuquerque, Oliveira Raquel de Vasconcellos Carvalhaes de, Perissé André Reynaldo Santos
Escola Nacional de Saúde Pública Sergio Arouca, Fundação Oswaldo Cruz, Rio de Janeiro, Brasil.
Centro Universitário Adventista de São Paulo, São Paulo, Brasil.
Cad Saude Publica. 2025 Apr 11;41(3):e00127424. doi: 10.1590/0102-311XPT127424. eCollection 2025.
The municipality of Rio de Janeiro is one of the most affected by the simultaneous circulation of the chikungunya (CHIKV), the Zika (ZIKV) and the dengue viruses (DENV) in Brazil. Although rapid tests are commercially available in the country, there are still doubts about their efficacy in flavivirus cocirculation scenarios. This this study aimed to evaluate the performance and best cut-off point of CHIKV rapid tests in a scenario of cocirculation of Zika and dengue in the Rio de Janeiro. A total of 2,120 volunteers who lived in permanent private households in Rio de Janeiro were included and tested for CHIKV using the rapid test Dual Path Platform (DPP). Of the total number of participants, 769 had venous blood samples collected for diagnostic confirmation by the gold standard plaque reduction neutralization test (PRNT) and tested for CHIKV, DENV and ZIKV. The receiver operating characteristic (ROC) curve was used to calculate sensitivity, specificity, positive and negative predictive values, and the best cut-off point. A total of 15.5% of the samples were identified as having previous exposure to CHIKV according to the rapid test and 20.4% of the samples had previous exposure to CHIKV according to the PRNT. We identified prior exposure to DENV in 89.2% of the samples tested using PRNT and 67.8% to ZIKV. The sensitivity and specificity found for the manufacturer's cut-off point was 96.1% and 97.5%, respectively. The best cut-off point found for the rapid test was ≥ 14, a result for which the accuracy was 97.7%, with specificity and sensitivity of 97.9% and 96.8%, respectively. It was concluded that the rapid test has high performance to detect CHIKV infection in a scenario of cocirculation of Zika and dengue.
里约热内卢市是巴西受基孔肯雅病毒（CHIKV）、寨卡病毒（ZIKV）和登革热病毒（DENV）同时传播影响最严重的地区之一。尽管该国已有快速检测试剂上市销售，但对于它们在黄病毒共同传播情况下的效力仍存在疑问。本研究旨在评估里约热内卢寨卡和登革热共同传播情况下CHIKV快速检测试剂的性能及最佳临界值。总共纳入了2120名居住在里约热内卢常住私人家庭的志愿者，使用双路径平台（DPP）快速检测试剂对其进行CHIKV检测。在所有参与者中，769人采集了静脉血样本，通过金标准蚀斑减少中和试验（PRNT）进行诊断确认，并检测CHIKV、DENV和ZIKV。采用受试者工作特征（ROC）曲线计算灵敏度、特异性、阳性和阴性预测值以及最佳临界值。根据快速检测试剂，共有15.5%的样本被鉴定为曾接触过CHIKV；根据PRNT，20.4%的样本曾接触过CHIKV。我们发现，使用PRNT检测的样本中，89.2%曾接触过DENV，67.8%曾接触过ZIKV。对于制造商设定的临界值，所发现的灵敏度和特异性分别为96.1%和97.5%。快速检测试剂的最佳临界值为≥14，此结果的准确率为97.7%，特异性和灵敏度分别为97.9%和96.8%。得出的结论是，在寨卡和登革热共同传播的情况下，快速检测试剂在检测CHIKV感染方面具有较高性能。
