Chen M X, Zhou Z Y, Qing W, Li H, Zhou H W
Department of Microbiology,School of Public Health, Southern Medical University, Guangzhou 510515, China Department of Laboratory Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China.
Department of Laboratory Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China.
Zhonghua Yu Fang Yi Xue Za Zhi. 2021 Jul 6;55(7):867-874. doi: 10.3760/cma.j.cn112150-20210224-00184.
To investigate the characteristics of cervical microbiota in patients with HPV (Human Papillomavirus) infection, and to analyze the associations of cervical microbiota and HPV infection or cervicitis. 300 samples underwent HPV nucleic acid testing was collected in this case-control study from June 2019 to April 2020 in the Zhujiang Hospital of Southern Medical University, there were 150 cases allocated in HPV infection group (HPV+), and 150 cases of negative nucleic acid test were non-infectious Group (HPV-). Next-generation sequencing was used to sequence the V4 region of the bacterial 16S rRNA gene, and QIIME pipeline was used to analysis the microbiota composition of the two groups. Wilcoxon rank sum test and Kruskal-Wallis test were used to statistically analysis the differences of the microbiota between groups; and the α diversity and β diversity of the flora between groups were statistically analyzed by Adonis multivariate analysis of variance and Wilcoxon rank sum test. A total of 300 samples were analyzed in this study, of which 150 samples were HPV-positive and 150 samples were HPV-negative; among HPV-positive cases, 132 were infected by high-risk HPV (88.0%), and 18 were low-risk HPV infections (12.0%). The composition of the cervical microbiota were significantly different between the HPV+group and the HPV-group, which in the HPV+group, the α diversity of the cervical microbiota were significantly increased (Shannon index, W=8 174, <0.000 1; PD whole tree, W=8 887, =0.001 7). The β diversity of the two groups was significantly different (Binary Jaccard, =2.325 4, =0.042 0; Bray Curtis, =2.136 44, =0.044 0). The relative abundance of . and in the HPV+group sample decreased significantly (W=7 730, <0.000 1; W=8 979, =0.002 5), accompanied by enriched There was no significant difference in the composition of the cervical microbiota between high-risk HPV infection and low-risk HPV infection (=4.100 4, >0.05). In addition, cervicitis is significantly related to HPV infection (χ²=19.78, <0.000 1), the composition of cervical flora has similarity features in cervicitis and HPV infection samples. Compared with the normal group, the cervical microbiota of cervicitis with HPV infection is mainly enriched in The cervical microbiota of patients with HPV infection has a significant dysbiosis, with increased diversity and significant depletion of lactobacillus, accompanied by an increase in the abundance of pathogenic bacteria such as
为研究人乳头瘤病毒（HPV）感染患者宫颈微生物群的特征，并分析宫颈微生物群与HPV感染或宫颈炎之间的关联。在这项病例对照研究中，于2019年6月至2020年4月在南方医科大学珠江医院收集了300份进行HPV核酸检测的样本，其中150例被分配到HPV感染组（HPV+），150例核酸检测阴性的为非感染组（HPV-）。采用二代测序对细菌16S rRNA基因的V4区域进行测序，并使用QIIME软件分析两组的微生物群组成。采用Wilcoxon秩和检验和Kruskal-Wallis检验对两组微生物群的差异进行统计学分析；采用Adonis多变量方差分析和Wilcoxon秩和检验对两组菌群的α多样性和β多样性进行统计学分析。本研究共分析了300份样本，其中150份样本HPV呈阳性，150份样本HPV呈阴性；在HPV阳性病例中，132例感染高危HPV（88.0%），18例为低危HPV感染（12.0%）。HPV+组和HPV-组的宫颈微生物群组成存在显著差异，在HPV+组中，宫颈微生物群的α多样性显著增加（香农指数，W = 8174，P < 0.0001；系统发育多样性，W = 8887，P = 0.0017）。两组的β多样性存在显著差异（二元杰卡德距离，P = 2.3254，P = 0.0420；布雷-柯蒂斯距离，P = 2.13644，P = 0.0440）。HPV+组样本中[具体细菌名称1]和[具体细菌名称2]的相对丰度显著降低（W = 7730，P < 0.0001；W = 8979，P = 0.0025），同时[具体细菌名称3]富集。高危HPV感染和低危HPV感染的宫颈微生物群组成无显著差异（P = 4.1004，P > 0.05）。此外，宫颈炎与HPV感染显著相关（χ² = 19.78，P < 0.0001），宫颈炎和HPV感染样本中的宫颈菌群组成具有相似特征。与正常组相比，HPV感染合并宫颈炎患者的宫颈微生物群主要富集于[具体细菌名称4]。HPV感染患者的宫颈微生物群存在显著失调，多样性增加，乳酸杆菌显著减少，同时[具体病原菌名称]等病原菌的丰度增加。
需注意，原文中部分细菌名称未给出具体内容，翻译时用[具体细菌名称]表示。
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