Shanmugam Annaian, Kathiresan Kandasamy, Nayak Lakshman
Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai, Tamil Nadu 608 502, India.
Department of Marine Sciences, Berhampur University, Berhampur, Odisha 760007, India.
Biotechnol Rep (Amst). 2015 Nov 10;9:25-30. doi: 10.1016/j.btre.2015.10.007. eCollection 2016 Mar.
Chitosan is a commercially available derivative of chitin that has been extensively studied for its antimicrobial properties. In order to improve the water solubility and its biological activity, the chemical modification or derivatisation is attempted. In the present investigation, the chitosan prepared from the cuttlebone of was being chemically modified by reacting it with orthophosphoric acid so as to obtain phosphorylated chitosan. Then the chitosan and phosphorylated chitosan were structurally characterized through FT-IR spectroscopy. Further the antibacterial activity of chitosan and phosphorylated chitosan was tested against clinically isolated human pathogens (Gram-positive: sp., and and Gram-negative: , , , , , , sp. and ) by well diffusion method and the Minimum Inhibitory Concentration (MIC) was also calculated. The results of the present study suggests that the chitosan and phosphorylated chitosan has concentration dependent antibacterial activity with variation against several pathogenic human pathogenic bacterial strains which indicates their possible use as antibacterial agents.
壳聚糖是一种可商购的几丁质衍生物，因其抗菌特性而受到广泛研究。为了提高其水溶性及其生物活性，人们尝试进行化学改性或衍生化。在本研究中，通过将乌贼骨制备的壳聚糖与正磷酸反应进行化学改性，以获得磷酸化壳聚糖。然后通过傅里叶变换红外光谱对壳聚糖和磷酸化壳聚糖进行结构表征。此外，采用打孔扩散法测试了壳聚糖和磷酸化壳聚糖对临床分离的人类病原体（革兰氏阳性菌： 菌、 菌和 菌，革兰氏阴性菌： 菌、 菌、 菌、 菌、 菌、 菌、 菌和 菌）的抗菌活性，并计算了最低抑菌浓度（MIC）。本研究结果表明，壳聚糖和磷酸化壳聚糖具有浓度依赖性抗菌活性，对几种致病性人类病原菌菌株有不同影响，这表明它们有可能用作抗菌剂。
