Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai 608 502, Tamil Nadu, India; Institute of Marine Microbes and Ecospheres, State Key Laboratory of Marine Environmental Science Xiamen University, Xiamen 361 005, Fujian, China.
Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai 608 502, Tamil Nadu, India.
Int J Biol Macromol. 2015 Mar;74:93-102. doi: 10.1016/j.ijbiomac.2014.11.034. Epub 2014 Dec 6.
The present findings were aimed to develop an effective form of collagen-chitosan film to investigate the wound healing property in Wistar rats. Primarily, the acid-soluble collagen (ASC) isolated, structurally and physicochemically characterized. FT-IR and 1H NMR spectra were confirmed the collagen peptide crosslinks. The molecular weight of ASC subunits was about 86 kDa and 79 kDa, the proline and hydroxyproline content was 13.15% and 7.55%, respectively. In addition, collagen-chitosan films were successfully prepared in a controlled environment and characterized by FT-IR and DSC. In wound healing, the experimental animal model showed the increased wound size reduction in the CChF treated rats (96.25%) when compared to control rats (33.75%) followed by CF treated rats (65%) and ChF (55%). The histological results well supported these findings. It can be concluded that a better and faster epithelization was observed in the CChF group compared to the other groups.
本研究旨在开发一种有效的胶原-壳聚糖膜，以研究 Wistar 大鼠的伤口愈合特性。首先，分离并结构和物理化学表征酸溶性胶原（ASC）。FT-IR 和 1H NMR 光谱证实了胶原肽交联。ASC 亚基的分子量约为 86 kDa 和 79 kDa，脯氨酸和羟脯氨酸含量分别为 13.15%和 7.55%。此外，在受控环境中成功制备了胶原-壳聚糖膜，并通过 FT-IR 和 DSC 进行了表征。在伤口愈合方面，实验动物模型显示，与对照组（33.75%）相比，CChF 处理组（96.25%）的伤口面积减少更多，其次是 CF 处理组（65%）和 ChF 组（55%）。组织学结果很好地支持了这些发现。可以得出结论，与其他组相比，CChF 组观察到更好和更快的上皮化。
