Huang Cong, Zhang Xue, He Dongfeng, Wu Qiang, Tang Rui, Xing Longsheng, Liu Wanxue, Wang Wenkai, Liu Bo, Xi Yu, Yang Nianwan, Wan Fanghao, Qian Wanqiang
Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, China.
College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao, China.
Front Physiol. 2021 Jul 7;12:690185. doi: 10.3389/fphys.2021.690185. eCollection 2021.
Insect olfaction is vital for foraging, mating, host-seeking, and avoidance of predators/pathogens. In insects, odorant binding proteins (OBPs) are involved in transporting hydrophobic odor molecules from the external environment to receptor neurons. The codling moth, , one of the most destructive insect fruit pests, causes enormous economic losses. However, little is known about the number, variety, gains and losses, and evolution of genes in . Here we report the identification of 40 in , most (75%) of which are classic , using genomic and transcriptomic analyses. Two genes were lost in relative to possible distant ancestor in Lepidoptera lineage based on an analysis of gene gains and losses. The phylogenetic tree and chromosome location showed that the expansion of genes mainly resulted from tandem duplications, as the gene was duplicated twice along with loss of . Two positive selection sites of the gene were identified while other genes evolved under purifying selection. Our results provide fundamental knowledge of genes allowing further study of their function in .
昆虫嗅觉对于觅食、交配、寻找宿主以及躲避捕食者/病原体至关重要。在昆虫中，气味结合蛋白（OBPs）参与将疏水性气味分子从外部环境转运至受体神经元。苹果蠹蛾是最具破坏性的昆虫水果害虫之一，会造成巨大的经济损失。然而，关于苹果蠹蛾中OBP基因的数量、种类、增减情况及进化，人们了解甚少。在此，我们通过基因组和转录组分析报告了在苹果蠹蛾中鉴定出40个OBP基因，其中大部分（75%）是经典OBP基因。基于基因得失分析，相对于鳞翅目谱系中可能的远祖，苹果蠹蛾中有两个OBP基因丢失。系统发育树和染色体定位表明，OBP基因的扩增主要源于串联重复，因为OBP基因在其丢失的同时被重复了两次。我们鉴定出了OBP基因的两个正选择位点，而其他OBP基因则在纯化选择下进化。我们的结果提供了关于OBP基因的基础知识，有助于进一步研究其在苹果蠹蛾中的功能。
