Luangpraditkun Kunlathida, Tissot Marion, Joompang Anupong, Charoensit Pensri, Grandmottet François, Viyoch Jarupa, Viennet Céline
Department of Pharmaceutical Technology, Faculty of Pharmaceutical Sciences and Center of Excellence for Innovation in Chemistry, Naresuan University, Phitsanulok 65000, Thailand.
UMR 1098 RIGHT INSERM EFS BFC, DImaCell Imaging Resource Center, University of Bourgogne Franche-Comté, Besançon 25000, France.
Oxid Med Cell Longev. 2021 Jul 6;2021:5067957. doi: 10.1155/2021/5067957. eCollection 2021.
Natural substances have gained considerable attention for skin protection against UV light reactions. plant's heartwood extract is comprised of artocarpin as a major substance, already known for its interesting biological attributes as an antimicrobial, an anti-inflammatory, an antioxidant, and a melanogenesis inhibitor. The present work clarified the mechanism of natural artocarpin (NAR) with a purity of approximately 99% against the effects of UVB-induced HaCaT keratinocyte apoptosis. The indicated results showed that NAR suppresses free radical production (ROS and nitrite) and apoptosis-related molecule activation (caspase-3, p-p53, p-p38, and NF-B p65) and secretion (TNF-). Additionally, NAR prevented structural damages (nuclei condensation and fragmentation, apoptotic body formation, impaired cell adherence and round cell shape, disruption of F-actin filament, and clustering of cell death receptor CD95/Fas) and biophysical changes (plasma membrane rigidification). Thus, NAR acts directly from scavenging free radicals generated by UV and indirectly by suppressing morphological and biochemical UV-induced cell damages. Its biological effects are mainly attributed to antioxidant and antiapoptotic properties. Taken together, NAR could be considered as an effective natural product for photoprotective formulations.
天然物质在皮肤抵御紫外线反应的保护方面已引起了相当大的关注。植物的心材提取物以面包果宁作为主要成分，其作为抗菌、抗炎、抗氧化和黑色素生成抑制剂的有趣生物学特性已为人所知。本研究阐明了纯度约为99%的天然面包果宁（NAR）对UVB诱导的HaCaT角质形成细胞凋亡作用的机制。所示结果表明，NAR可抑制自由基产生（活性氧和亚硝酸盐）以及凋亡相关分子的激活（半胱天冬酶-3、磷酸化p53、磷酸化p38和核因子-κB p65）及分泌（肿瘤坏死因子-α）。此外，NAR可防止结构损伤（细胞核浓缩和碎片化、凋亡小体形成、细胞黏附受损和细胞圆形化、F-肌动蛋白丝破坏以及细胞死亡受体CD95/Fas聚集）和生物物理变化（质膜硬化）。因此，NAR通过清除紫外线产生的自由基直接发挥作用，并通过抑制紫外线诱导的细胞形态和生化损伤间接发挥作用。其生物学效应主要归因于抗氧化和抗凋亡特性。综上所述，NAR可被视为一种用于光保护制剂的有效天然产物。
