Misischia W Patrick, Xenoudi Pinelopi, Yukna Raymond A, Schurr Michael J
Advanced Periodontal Therapies, Department of Surgical Dentistry, University of Colorado School of Dental Medicine, Aurora, CO, USA.
, Boulder, CO, USA.
Lasers Med Sci. 2021 Oct;36(8):1759-1767. doi: 10.1007/s10103-021-03349-3. Epub 2021 Jul 27.
Compare the effectiveness of selected dental lasers for decontamination of machined titanium surfaces in vitro. Seventy-two sterile machined surface titanium discs were individually inoculated with strains of Streptococcus mutans (Sm), Streptococcus oralis (So), Aggregatibacter actinomycetemcomitans (Aa), or all three bacteria together (MIX) at 34.0 C, 20.8% O and 5% CO for 12 h. After incubation, the discs were divided into six groups: 1) no treatment, 2) 0.12% chlorhexidine gluconate (CHX), and 3) 10,600 CO, 4) 810 nm diode, 5) 2780 nm Er,Cr:YSGG, 6) 1064 nm Nd:YAG laser groups. After treatment, any remaining viable bacteria were liberated from the discs via sonication, transferred onto brain heart infusion (BHI) agar plates for culturing, and colony-forming units (CFUs) were recorded. Statistical analysis was performed. There were statistically significantly differences (SSD) (p < 0.01) in bacterial reduction of discs individually inoculated with Aa between the Er,Cr:YSGG and Nd:YAG lasers. There was also a SSD (p < 0.01) lower effect with the MIX with the Er,Cr:YSGG compared with all other modalities. Bacterial reduction with the CO was better (p < 0.001) than treatment with CHX or the Er,Cr:YSGG laser on killing of So. Although all modalities of treatment showed a mean of 98% or greater viable bacterial reduction, the most consistent bacterial reduction of all titanium discs was with the Nd:YAG laser (100%).
比较所选牙科激光在体外对加工钛表面进行去污的效果。将72个无菌加工表面钛盘分别接种变形链球菌（Sm）、口腔链球菌（So）、伴放线聚集杆菌（Aa）菌株或三种细菌混合菌（MIX），在34.0℃、20.8%氧气和5%二氧化碳环境下培养12小时。培养后，将圆盘分为六组：1）不处理；2）0.12%葡萄糖酸氯己定（CHX）；3）10600 CO；4）810纳米二极管；5）2780纳米铒铬：钇钪镓石榴石（Er,Cr:YSGG）；6）1064纳米钕：钇铝石榴石（Nd:YAG）激光组。处理后，通过超声处理从圆盘中释放出任何残留的活菌，转移到脑心浸液（BHI）琼脂平板上进行培养，并记录菌落形成单位（CFU）。进行了统计分析。在单独接种Aa的圆盘的细菌减少方面，铒铬：钇钪镓石榴石激光和钕：钇铝石榴石激光之间存在统计学显著差异（SSD）（p < 0.01）。与所有其他方式相比，铒铬：钇钪镓石榴石激光对混合菌的效果也有统计学显著差异（p < 0.01）且较低。在杀死So方面，CO激光的细菌减少效果比CHX或铒铬：钇钪镓石榴石激光处理更好（p < 0.001）。尽管所有处理方式均显示活菌减少平均值达到98%或更高，但所有钛盘最一致的细菌减少是使用钕：钇铝石榴石激光（100%）。
