医院环境中使用的汽化过氧化氢消毒可灭活新型冠状病毒（SARS-CoV-2）和人冠状病毒229E（HCoV-229E），且不会降低未过期N95口罩的过滤效率。
Vapourized hydrogen peroxide decontamination in a hospital setting inactivates SARS-CoV-2 and HCoV-229E without compromising filtration efficiency of unexpired N95 respirators.
作者信息
Christie-Holmes Natasha, Tyli Rachel, Budylowski Patrick, Guvenc Furkan, Weiner Amit, Poon Betty, Speck Mary, Naugler Stephenie, Rainville Allen, Ghalami Ayoob, McCaw Shannon, Hayes Steven, Mubareka Samira, Gray-Owen Scott D, Rotstein Ori D, Kandel Rita A, Scott James A
机构信息
Combined Containment Level 3 Unit, Temerty Faculty of Medicine, University of Toronto, Toronto, ON Canada M5S 1A8.
Dalla Lana School of Public Health, University of Toronto, 223 College Street, Toronto, ON, Canada, M5T 1R4.
出版信息
Am J Infect Control. 2021 Oct;49(10):1227-1231. doi: 10.1016/j.ajic.2021.07.012. Epub 2021 Jul 25.
BACKGROUND
The COVID-19 pandemic highlighted the need for evidence-based approaches to decontamination and reuse of N95 filtering facepiece respirators (FFRs). We sought to determine whether vapourized hydrogen peroxide (VHP) reduced SARS-CoV-2 bioburden on FFRs without compromising filtration efficiency. We also investigated coronavirus HCoV-229E as a surrogate for decontamination validation testing.
METHODS
N95 FFRs were laced with SARS-CoV-2 or HCoV-229E and treated with VHP in a hospital reprocessing facility. After sterilization, viral burden was determined using viral outgrowth in a titration assay, and filtration efficiency of FFRs was tested against ATSM F2299 and NIOSH TEB-STP-APR-0059.
RESULTS
Viable SARS-CoV-2 virus was not detected after VHP treatment. One replicate of the HCoV-229E laced FFRs yielded virus after processing. Unexpired N95 FFRs retained full filtration efficiency after VHP processing. Expired FFRs failed to meet design-specified filtration efficiency and therefore are unsuitable for reprocessing.
DISCUSSION
In-hospital VHP is an effective decontaminant for SARS-CoV-2 on FFRs. Further, filtration efficiency of unexpired respirators is not affected by this decontamination process.
CONCLUSIONS
VHP is effective in inactivating SARS-CoV-2 on FFRs without compromising filtration efficiency. HCoV-229E is a suitable surrogate for SARS-CoV-2 for disinfection studies.
背景
2019冠状病毒病大流行凸显了对N95过滤式面罩呼吸器（FFR）进行基于证据的去污和再利用方法的需求。我们试图确定汽化过氧化氢（VHP）是否能降低FFR上的严重急性呼吸综合征冠状病毒2（SARS-CoV-2）生物负荷，同时又不影响过滤效率。我们还研究了冠状病毒HCoV-229E作为去污验证测试的替代物。
方法
在医院的再处理设施中，将SARS-CoV-2或HCoV-229E接种到N95 FFR上，并用VHP进行处理。灭菌后，通过滴定试验中的病毒生长来确定病毒负荷，并根据美国材料与试验协会（ASTM）F2299和美国国家职业安全与健康研究所（NIOSH）TEB-STP-APR-0059对FFR的过滤效率进行测试。
结果
VHP处理后未检测到活的SARS-CoV-2病毒。一组接种HCoV-229E的FFR在处理后产生了病毒。未过期的N95 FFR在VHP处理后保持了完全的过滤效率。过期的FFR未能达到设计规定的过滤效率，因此不适合再处理。
讨论
医院内的VHP是FFR上SARS-CoV-2的有效去污剂。此外，未过期呼吸器的过滤效率不受此去污过程的影响。
结论
VHP能有效灭活FFR上的SARS-CoV-2，且不影响过滤效率。HCoV-229E是SARS-CoV-2消毒研究的合适替代物。
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