Department of Forest Resource Chemistry, Forestry and Forest Products Research Institute, Tsukuba, Ibaraki 305-8687 Japan.
Department of Forest Resource Chemistry, Forestry and Forest Products Research Institute, Tsukuba, Ibaraki 305-8687 Japan.
Bioresour Technol. 2021 Oct;337:125489. doi: 10.1016/j.biortech.2021.125489. Epub 2021 Jul 2.
Vanillin and vanillate are the major lignin-derived aromatic compounds produced through the alkaline oxidation of softwood lignin. Because the production of higher-value added chemicals from these compounds is essential for lignin valorization, the microbial production of β-ketoadipate, a promising raw material for the synthesis of novel nylons, from lignin was considered. Pseudomonas putida KT2440 was engineered to convert vanillin and vanillate to β-ketoadipate. By examining the culture conditions with an initial culture volume of 1 L, the engineered strain completely converted 25 g of vanillin and 25 g of vanillate and produced approximately 23 g of β-ketoadipate from each of them with a yield of 93% or higher. Furthermore, this strain showed the ability to efficiently produce β-ketoadipate from softwood lignin extracts in black liquor, a byproduct of pulp production. These results suggest that the production of β-ketoadipate from industrial black liquor is highly feasible for substantial lignin valorization.
香草醛和香草酸盐是主要的木质素衍生芳香族化合物，通过软木木质素的碱性氧化产生。由于这些化合物的高附加值化学品的生产对于木质素的增值至关重要，因此考虑了从木质素微生物生产β-酮戊二酸，这是合成新型尼龙的有前途的原料。对恶臭假单胞菌 KT2440 进行了工程改造，以将香草醛和香草酸盐转化为β-酮戊二酸。通过在初始培养体积为 1 L 的情况下检查培养条件，该工程菌株完全转化了 25 g 香草醛和 25 g 香草酸盐，并从每一种中产生了大约 23 g 的β-酮戊二酸，产率在 93%或更高。此外，该菌株表现出从纸浆生产的副产品黑液中的软木木质素提取物中有效生产β-酮戊二酸的能力。这些结果表明，从工业黑液中生产β-酮戊二酸对于木质素的大量增值是高度可行的。
