Int J Periodontics Restorative Dent. 2021 Jul-Aug;41(4):589-597. doi: 10.11607/prd.4448.
This study assessed the levels of tumor necrosis factor-α (TNF-α), prostaglandin E2 (PGE2), receptor activator of nuclear factor kappa B (RANK), RANK ligand (RANKL), osteoprotegerin (OPG), and levels of Fusobacterium nucleatum, Porphyromonas gingivalis, Treponema denticola, Tannerella forsythia, Prevotella intermedia, and Streptococcus oralis in areas where airborne particle-abraded, large-grit, acid-etched (SLA), fluorine-modified, and anodized implant surfaces are used. A total of 71 implants from 37 patients were assessed, grouped according to the surface characteristics of the implants: SLA surface (Group 1), fluorine-modified surface (Group 2), and anodized surface (Group 3). The following clinical indices were measured: Gingival Index (GI), probing depth (PD), bleeding on probing (BOP), clinical attachment level (CAL), and keratinized tissue width (KTW). Peri-implant sulcus fluid and subgingival plaque samples were also collected. Commercial enzyme-linked immunosorbent assay (ELISA) kits were purchased for measuring TNF-α, PGE2, RANKL, RANK, and OPG. Real-time quantitative polymerase chain reaction (PCR) was used to detect P intermedia, T forsythia, T denticola, F nucleatum, P gingivalis, and S oralis levels in the subgingival biofilms. The groups showed no statistically significant differences in GI, PD, BOP, CAL, KTW, or peri-implant status. The total amounts of PGE2, TNF-α, RANKL, RANK, and OPG and the RANKL/OPG ratio were not significantly different between groups. F nucleatum, T forsythia, P intermedia, P gingivalis, and T denticola were significantly higher in Group 3 implants. DNA concentrations of S oralis were higher in Group 2. Within the limitations of this study, SLA and fluorine-modified implant surfaces may be more clinically successful than anodized-surface implants.
本研究评估了使用空气颗粒磨损、大粒度酸蚀（SLA）、氟改性和阳极氧化植入物表面的情况下，肿瘤坏死因子-α（TNF-α）、前列腺素 E2（PGE2）、核因子κ B 受体激活剂（RANK）、RANK 配体（RANKL）、骨保护素（OPG）以及核梭杆菌、牙龈卟啉单胞菌、牙髓卟啉单胞菌、福赛斯坦纳菌、中间普氏菌和口腔链球菌水平。评估了来自 37 名患者的总共 71 个植入物，根据植入物的表面特征进行分组：SLA 表面（第 1 组）、氟改性表面（第 2 组）和阳极氧化表面（第 3 组）。测量了以下临床指标：牙龈指数（GI）、探诊深度（PD）、探诊出血（BOP）、临床附着水平（CAL）和角化组织宽度（KTW）。还收集了种植体周沟液和龈下菌斑样本。购买了商业酶联免疫吸附测定（ELISA）试剂盒来测量 TNF-α、PGE2、RANKL、RANK 和 OPG。实时定量聚合酶链反应（PCR）用于检测龈下生物膜中的中间普氏菌、福赛斯坦纳菌、牙髓卟啉单胞菌、核梭杆菌、牙龈卟啉单胞菌和口腔链球菌水平。各组在 GI、PD、BOP、CAL、KTW 或种植体周围状况方面没有统计学上的显著差异。RANKL/OPG 比值在各组之间没有显著差异。PGE2、TNF-α、RANKL、RANK 和 OPG 的总量在各组之间没有显著差异。核梭杆菌、福赛斯坦纳菌、中间普氏菌、牙龈卟啉单胞菌和牙髓卟啉单胞菌在第 3 组植入物中显著更高。第 2 组口腔链球菌的 DNA 浓度更高。在本研究的限制范围内，SLA 和氟改性植入物表面可能比阳极氧化植入物更具临床成功性。
