Korean Institute of Tuberculosis, Osong, Cheongju-si, Chungcheongbuk-do, 28158, Republic of Korea.
QuantaMatrix Inc., Seoul National Hospital CMI, Jongno-gu, Seoul, 03082, Republic of Korea.
Appl Microbiol Biotechnol. 2021 Aug;105(14-15):6059-6072. doi: 10.1007/s00253-021-11446-0. Epub 2021 Jul 30.
The QuantaMatrix Microfluidic Agarose Channel (QMAC) system was used for rapid drug susceptibility testing (DST). Here, we performed DST using QMAC integrated with the mycobacteria growth indicator tube (MGIT) liquid culture employing a specially designed cross agarose channel for the tuberculosis chip. MGIT-, QMAC-, and Löwenstein-Jensen (LJ)-DSTs were performed using 13 drugs. The protocol for QMAC-DST was optimized using the inoculum obtained after the disaggregation of Mycobacterium tuberculosis clumps in MGIT culture. The completion times of QMAC-DST and MGIT-DST were analyzed, and the results of all three DSTs were compared. Discrepant results were analyzed using line probe assays and DNA sequencing. Nontuberculous mycobacteria were distinguished using the ρ-nitrobenzoic acid inhibition test. The overall agreement rate of QMAT-DST and LJ-DST was 97.0% and that of QMAT-DST and MGIT-DST was 86.3%. An average turnaround time for DST was 5.4 days, which was considerably less than the time required for MGIT-DST. The overall time required to obtain DST results using QMAC-DST integrated with MGIT culture was an average of 18.6 days: 13.2 days for culture and identification and 5.4 days for DST. Hence, QMAC-DST integrated with liquid culture can be used to perform DSTs with short turnaround times and effective detection. KEY POINTS: • QMAC system can simultaneously perform phenotypic DST with 13 anti-TB drugs and PNB. • An optimized DST protocol led to a marked decrease in clumping in MGIT culture. • QMAC system integrated with MGIT liquid culture system reduced the turnaround time.
量子矩阵琼脂糖通道（QMAC）系统用于快速药敏试验（DST）。在这里，我们使用 QMAC 与分枝杆菌生长指示剂管（MGIT）液体培养物结合进行 DST，使用专为结核芯片设计的交叉琼脂糖通道。使用 13 种药物进行 MGIT、QMAC 和 Löwenstein-Jensen（LJ）-DST。使用 MGIT 培养物中分枝杆菌聚集体的离散接种物优化 QMAC-DST 方案。分析 QMAC-DST 和 MGIT-DST 的完成时间，并比较所有三种 DST 的结果。使用线探针分析和 DNA 测序分析不一致的结果。使用 ρ-硝基苯甲酸抑制试验区分非结核分枝杆菌。QMAT-DST 和 LJ-DST 的总符合率为 97.0%，QMAT-DST 和 MGIT-DST 的总符合率为 86.3%。DST 的平均周转时间为 5.4 天，明显短于 MGIT-DST 所需的时间。使用 QMAC 与 MGIT 培养物结合进行 DST 的总时间平均为 18.6 天：培养和鉴定需要 13.2 天，DST 需要 5.4 天。因此，QMAC 与液体培养物结合的 DST 可以在短周转时间内进行有效的检测。要点：• QMAC 系统可以同时进行 13 种抗结核药物和 PNB 的表型 DST。• 优化的 DST 方案导致 MGIT 培养物中聚集体明显减少。• QMAC 系统与 MGIT 液体培养系统相结合，缩短了周转时间。
