Division of Pulmonary and Critical Care Medicine, Department of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea.
Ann Lab Med. 2012 Jul;32(4):264-9. doi: 10.3343/alm.2012.32.4.264. Epub 2012 Jun 20.
The purpose of this study was to compare the turnaround time for liquid culturing and primary anti-tuberculous drug susceptibility testing (DST) performed using the mycobacteria growth indicator tube (MGIT) 960 system (Becton Dickinson, USA) with that for conventional culturing and DST (by the absolute concentration method) performed using solid culture medium and to determine the concordance rates of DST results obtained using these 2 methods.
In this retrospective study, we compared the turnaround times from receiving the request for mycobacterial culture to reporting the DST results before and after the introduction of the MGIT 960 system. Further, we determined the concordance between DST results for isoniazid and rifampin for Mycobacterium tuberculosis isolates obtained using the MGIT 960 system and the absolute concentration method, which was conducted at the Korean Institute of Tuberculosis.
The overall turnaround time for mycobacterial culturing and DST was 27 days for liquid culturing and DST using the MGIT 960 system versus approximately 70 days for culturing on solid medium and DST with the absolute concentration method (P<0.001). There was a good concordance between findings of DST obtained with the 2 methods (97.2%, kappa coefficient=0.855 for rifampin; and 95.6%, kappa coefficient=0.864 for isoniazid), for 1,083 clinical isolates.
The automated MGIT 960 system for culturing and DST of M. tuberculosis was successfully introduced in a hospital laboratory setting in Korea with significant shortening of the turnaround time.
本研究旨在比较使用分枝杆菌生长指示管（MGIT）960 系统（美国 Becton Dickinson）进行液体培养和初步抗结核药物敏感性试验（DST）与使用固体培养基进行常规培养和 DST（绝对浓度法）的周转时间，并确定这两种方法获得的 DST 结果的一致性率。
在这项回顾性研究中，我们比较了引入 MGIT 960 系统前后从收到分枝杆菌培养请求到报告 DST 结果的周转时间。此外，我们还确定了在韩国结核研究所使用 MGIT 960 系统和绝对浓度法获得的结核分枝杆菌分离株异烟肼和利福平的 DST 结果之间的一致性。
液体培养和 MGIT 960 系统 DST 的分枝杆菌培养和 DST 的总周转时间为 27 天，而固体培养基培养和绝对浓度法 DST 约为 70 天（P<0.001）。两种方法的 DST 结果之间存在良好的一致性（利福平的一致性为 97.2%，kappa 系数=0.855；异烟肼的一致性为 95.6%，kappa 系数=0.864），共 1083 例临床分离株。
在韩国的一家医院实验室环境中，成功引入了用于培养和检测结核分枝杆菌的自动化 MGIT 960 系统，显著缩短了周转时间。
