College of Chemistry and Chemical Engineering, Southwest Petroleum University, Chengdu 610500, People's Republic of China; State Key Laboratory of Oil & Gas Reservoir Geology and Exploitation, Southwest Petroleum University, Chengdu 610500, People's Republic of China.
College of Chemistry and Chemical Engineering, Southwest Petroleum University, Chengdu 610500, People's Republic of China.
Bioorg Chem. 2021 Sep;114:105020. doi: 10.1016/j.bioorg.2021.105020. Epub 2021 May 26.
With the development of frame materials, metal organic frameworks (MOFs) have been successfully applied in the fields of biological small molecule analysis and fluorescent DNA detection. In this work, in view of the good adsorption characteristics of MIL-101(Cr), the highly sensitive detection of adenosine triphosphate (ATP) assisted nucleic acid exonuclease amplification by MIL-101(Cr) on the different affinity of single stranded DNA and double stranded DNA was investigated. The detection limit of ATP reaches 1.7 μM, and the platform has good applicability in biological samples. On this basis, an "AND" logic gate was successfully constructed. Superior sensitivity to ATP in the presence of exonuclease was reflected, which greatly enhanced the system's fluorescence. Importantly, the fluorescence sensing application of this nanomaterial inspired other target detection and enriched the building blocks of fluorescence sensing platform.
随着框架材料的发展，金属有机骨架（MOFs）已成功应用于生物小分子分析和荧光 DNA 检测领域。在这项工作中，鉴于 MIL-101（Cr）良好的吸附特性，通过 MIL-101（Cr）对单链 DNA 和双链 DNA 不同亲和力的高度敏感检测，实现了对三磷酸腺苷（ATP）的辅助核酸外切酶扩增的高灵敏度检测。ATP 的检测限达到 1.7 μM，该平台在生物样品中具有良好的适用性。在此基础上，成功构建了“与”逻辑门。在外切酶存在的情况下对 ATP 表现出优异的灵敏度，大大增强了系统的荧光。重要的是，这种纳米材料的荧光传感应用激发了其他目标检测，并丰富了荧光传感平台的构建模块。
