Welburn S C, Arnold K, Maudlin I, Gooday G W
Tsetse Research Laboratory, ODA/University of Bristol, Langford.
Parasitology. 1993 Aug;107 ( Pt 2):141-5. doi: 10.1017/s003118200006724x.
Rickettsia-like organisms (RLO) from testse midguts and mosquito cell cultures showed high levels of endochitinase activity. A line of Glossina morsitans morsitans highly susceptible to midgut trypanosome infection and with high incidence of RLO infection showed significantly greater chitinolytic activity than G. austeni which had low RLO incidence and were correspondingly refractory to midgut infection. Midgut infection rates of Trypanosoma brucei rhodesiense in G. m. morsitans showed a dose-related increase when flies were fed N-acetyl-D-glucosamine (GlcNAc) in the infective meal and for 4 subsequent days. A model is proposed for susceptibility to trypanosome infection based on the generation of GlcNAc by RLO endochitinase activity in tsetse pupae inhibiting midgut lectin in teneral flies.
采采蝇中肠和蚊子细胞培养物中的类立克次氏体生物（RLO）表现出高水平的内切几丁质酶活性。一组对中肠锥虫感染高度敏感且RLO感染发生率高的 morsitans morsitans采采蝇，其几丁质分解活性明显高于RLO发生率低且对中肠感染相应具有抗性的 austeni采采蝇。当在感染性食物中给m. morsitans采采蝇喂食N-乙酰-D-葡萄糖胺（GlcNAc）并在随后4天持续喂食时，罗得西亚布氏锥虫在其体内的中肠感染率呈剂量相关增加。基于采采蝇蛹中RLO内切几丁质酶活性产生的GlcNAc抑制 teneral 采采蝇中肠凝集素，提出了一个采采蝇对锥虫感染易感性的模型。
