The Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education, Chinese National Health Commission and Chinese Academy of Medical Sciences, The State and Shandong Province Joint Key Laboratory of Translational Cardiovascular Medicine, Department of Cardiology, 16275991623Qilu Hospital of Shandong University, Jinan, Shandong, China.
Department of Pulmonary and Critical Care Medicine, Yantai Yuhuangding Hospital, Yantai, Shandong, P.R. China.
Angiology. 2022 Jul;73(6):579-587. doi: 10.1177/00033197211034286. Epub 2021 Jul 30.
Circulating levels of microRNA-221 and 222 (miR-221/222) in patients with coronary artery disease (CAD) are elevated, yet the relationship between circulating miR-221/222 and the severity of coronary lesions in patients with acute coronary syndrome (ACS) remains unknown. In this study, the relative expression levels of circulating miR-221/222 in patients with ACS (n = 267) and controls (n = 71) were compared by real-time fluorescence quantitative-polymerase chain reaction (RT-qPCR). The ACS group was further divided into unstable angina pectoris (UA) group (n = 191) and acute myocardial infarction (AMI) group (n = 76). Significant upregulation of circulating miR-221/222 was observed in ACS. A positive linear correlation between circulating miR-221/222 and Gensini scores was demonstrated. The area under the curve (AUC) of circulating miR-221/222 in the diagnosis of coronary artery stenosis ≥50% was 0.605 and 0.643, respectively. The circulating miRNA-221/222 expression levels in ACS patients were elevated and positively associated with the severity of the coronary artery lesions. Circulating miR-221/222 may be novel biomarkers for the diagnosis of coronary artery stenosis ≥50% and the occurrence of ACS.
循环 microRNA-221 和 222（miR-221/222）水平在冠心病（CAD）患者中升高，但急性冠脉综合征（ACS）患者循环 miR-221/222 与冠状动脉病变严重程度之间的关系尚不清楚。在这项研究中，通过实时荧光定量聚合酶链反应（RT-qPCR）比较了 ACS 患者（n=267）和对照组（n=71）的循环 miR-221/222 相对表达水平。ACS 组进一步分为不稳定型心绞痛（UA）组（n=191）和急性心肌梗死（AMI）组（n=76）。ACS 患者的循环 miR-221/222 显著上调。循环 miR-221/222 与 Gensini 评分之间存在正线性相关。循环 miR-221/222 诊断冠状动脉狭窄≥50%的曲线下面积（AUC）分别为 0.605 和 0.643。ACS 患者的循环 miRNA-221/222 表达水平升高，与冠状动脉病变的严重程度呈正相关。循环 miR-221/222 可能是诊断冠状动脉狭窄≥50%和 ACS 发生的新型生物标志物。
