Department of Cardiology, The 960th Hospital of The Joint Service Support Force of The People's Liberation Army, Jinan, Shandong 250031, P.R. China.
Department of Cardiology, The 960th Hospital of The Joint Service Support Force of The People's Liberation Army, Jinan, Shandong 250031, P.R. China.
Mol Med Rep. 2023 Nov;28(5). doi: 10.3892/mmr.2023.13090. Epub 2023 Sep 15.
Exosomal microRNAs (miRNAs/miRs) are potential biomarkers for the diagnosis and treatment of cardiovascular disease, and hyperglycemia serves an important role in the development of atherosclerosis. The present study aimed to investigate the expression profile of serum‑derived exosomal miRNAs in coronary heart disease (CHD) with hyperglycemia, and to identify effective biomarkers for predicting coronary artery lesions. Serum samples were collected from eight patients with CHD and hyperglycemia and eight patients with CHD and normoglycemia, exosomes were isolated and differentially expressed miRNAs (DEMIs) were filtered using a human miRNA microarray. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were performed using standard enrichment computational methods for the target genes of DEMIs. Receiver operating characteristic (ROC) curve analysis was applied to evaluate the values of the selected DEMIs in predicting the severity of coronary stenosis. A total of 10 DEMIs, including four upregulated miRNAs (hsa‑let‑7b‑5p, hsa‑miR‑4313, hsa‑miR‑4665‑3p and hsa‑miR‑940) and six downregulated miRNAs (hsa‑miR‑4459, hsa‑miR‑4687‑3p, hsa‑miR‑6087, hsa‑miR‑6089, hsa‑miR‑6740‑5p and hsa‑miR‑6800‑5p), were screened in patients with CHD and hyperglycemia. GO analysis showed that the 'cellular process', 'single‑organism process' and 'biological regulation' were significantly enriched. KEGG pathway analysis revealed that the 'mTOR signaling pathway', 'FoxO signaling pathway' and 'neurotrophin signaling pathway' were significantly enriched. Among these DEMIs, only hsa‑let‑7b‑5p expression was positively correlated with both hemoglobin A1C levels and Synergy between Percutaneous Coronary Intervention with Taxus and Cardiac Surgery score. ROC curves showed that hsa‑let‑7b‑5p could serve as an effective biomarker for differentiating the severity of coronary stenosis. In conclusion, the present study demonstrated that serum‑derived exosomal hsa‑let‑7b‑5p is upregulated in patients with CHD and hyperglycemia, and may serve as a noninvasive biomarker for the severity of coronary stenosis.
外泌体 microRNAs（miRNAs/miRs）是心血管疾病诊断和治疗的潜在生物标志物，而高血糖在动脉粥样硬化的发生中起着重要作用。本研究旨在探讨冠心病伴高血糖患者血清衍生外泌体 miRNAs 的表达谱，并寻找预测冠状动脉病变的有效生物标志物。收集了 8 例冠心病伴高血糖和 8 例冠心病伴正常血糖患者的血清样本，分离外泌体并使用人类 miRNA 微阵列筛选差异表达的 miRNAs（DEMIs）。使用标准富集计算方法对 DEMIs 的靶基因进行基因本体论（GO）和京都基因与基因组百科全书（KEGG）通路分析。应用受试者工作特征（ROC）曲线分析评估所选 DEMIs 预测冠状动脉狭窄严重程度的价值。共筛选出 10 个 DEMIs，包括 4 个上调 miRNA（hsa-let-7b-5p、hsa-miR-4313、hsa-miR-4665-3p 和 hsa-miR-940）和 6 个下调 miRNA（hsa-miR-4459、hsa-miR-4687-3p、hsa-miR-6087、hsa-miR-6089、hsa-miR-6740-5p 和 hsa-miR-6800-5p）。GO 分析显示，“细胞过程”、“单一生物体过程”和“生物调节”显著富集。KEGG 通路分析显示，“mTOR 信号通路”、“FoxO 信号通路”和“神经营养因子信号通路”显著富集。在这些 DEMIs 中，只有 hsa-let-7b-5p 的表达与血红蛋白 A1C 水平和经皮冠状动脉介入与心脏手术评分之和呈正相关。ROC 曲线显示，hsa-let-7b-5p 可作为区分冠状动脉狭窄严重程度的有效生物标志物。综上所述，本研究表明，冠心病伴高血糖患者血清源性外泌体 hsa-let-7b-5p 上调，可能作为冠状动脉狭窄严重程度的无创性生物标志物。
