Ling Hao, Guo Ziyuan, Shi Yongfeng, Zhang Lei, Song Chunli
Department of Cardiology, The Second Hospital of Jilin University, Changchun, China.
Department of Neurology, The Second Hospital of Jilin University, Changchun, China.
Front Physiol. 2020 Jun 11;11:654. doi: 10.3389/fphys.2020.00654. eCollection 2020.
Acute coronary syndrome (ACS) is a serious threat to public health. Based on clinical manifestations, ACS can be classified into unstable angina (UA) pectoris and acute myocardial infarction (AMI). The purpose of this study was to explore the possibility of using serum exosomal microRNA (miR)-126, miR-21, and phosphatase and tensin homolog (PTEN) expression levels as biomarkers of UA and AMI and to investigate whether these levels were positively correlated with the severity of coronary stenosis based on the Gensini score. Exosomes were isolated by ultracentrifugation from the serum of 34 patients with AMI, 31 patients with UA, and 22 healthy controls. The isolated exosomes were characterized by electron microscopy and particle size analysis; exosomal identity was further confirmed by western blotting using exosome-specific antibodies. Real-time quantitative polymerase chain reaction indicated that the serum exosomal levels of miR-126 and miR-21 were significantly higher in the patients with UA and AMI than in the healthy controls. Enzyme-linked immunosorbent assay showed that the serum exosomal PTEN levels were significantly higher in the UA and AMI groups than in the control group. Receiving operating characteristic curve analysis demonstrated the diagnostic efficiency of serum exosomal miR-126, miR-21, and PTEN levels for predicting AMI and UA. In addition, the circulating exosomal miR-126 level was positively correlated with the severity of coronary artery stenosis in patients with UA and AMI based on the Gensini score.
急性冠状动脉综合征（ACS）是对公众健康的严重威胁。根据临床表现，ACS可分为不稳定型心绞痛（UA）和急性心肌梗死（AMI）。本研究的目的是探讨使用血清外泌体微小RNA（miR）-126、miR-21和磷酸酶及张力蛋白同源物（PTEN）表达水平作为UA和AMI生物标志物的可能性，并基于Gensini评分研究这些水平是否与冠状动脉狭窄的严重程度呈正相关。通过超速离心从34例AMI患者、31例UA患者和22例健康对照者的血清中分离出外泌体。通过电子显微镜和粒径分析对分离出的外泌体进行表征；使用外泌体特异性抗体通过蛋白质印迹进一步确认外泌体的身份。实时定量聚合酶链反应表明，UA和AMI患者血清外泌体中miR-126和miR-21的水平显著高于健康对照者。酶联免疫吸附测定显示，UA和AMI组血清外泌体PTEN水平显著高于对照组。受试者工作特征曲线分析证明了血清外泌体miR-126、miR-21和PTEN水平对预测AMI和UA的诊断效率。此外，基于Gensini评分，UA和AMI患者循环外泌体miR-126水平与冠状动脉狭窄的严重程度呈正相关。
