Department of Immunology, Genetics and Pathology, Uppsala University, Uppsala, Sweden.
Methods Mol Biol. 2021;2350:125-144. doi: 10.1007/978-1-0716-1593-5_9.
In this chapter, we describe the pipeline for multiplex immunohistochemical staining, multispectral image acquisition, and analysis. The protocol is dedicated for use on human formalin fixed paraffin embedded (FFPE) tissues and utilizes immune markers of dendritic cells, myeloid cells, and macrophages, as well as cytokeratin. This provides quantitative data of the (co-)expression levels and spatial localization of immune cell subtypes.
在本章中，我们描述了多重免疫组化染色、多光谱图像采集和分析的工作流程。该方案专门针对福尔马林固定石蜡包埋（FFPE）组织中的人类组织使用，并利用树突状细胞、髓样细胞和巨噬细胞以及细胞角蛋白的免疫标志物。这提供了免疫细胞亚型的（共）表达水平和空间定位的定量数据。
