Marafioti Teresa, Lozano Maria D, de Andrea Carlos E
Department of Histopathology, University College London, London, United Kingdom; Department of Cellular Pathology, University College Hospital, London, United Kingdom.
Department of Pathology, Clínica Universidad de Navarra, University of Navarra, Pamplona, Spain; Department of Anatomy, Physiology and Pathology, University of Navarra, Pamplona, Spain.
Methods Cell Biol. 2023;174:43-53. doi: 10.1016/bs.mcb.2022.07.003. Epub 2022 Sep 6.
Multiplexed immunofluorescence imaging of formalin-fixed, paraffin-embedded (FFPE) specimens mounted on glass slides allow the identification of multiple cell phenotypes while retaining spatial and morphological context. Multiplex immunofluorescence protocols have already been developed and validated for mouse tissues. Immunophenotyping analysis reliably depicts the immune landscape of cancer tissues that has been demonstrated to influence cancer development and progression as well as to have an impact on therapy responsiveness and resistance. Here, we describe a method for multiplexed fluorescence image analysis, enabling analysis of mouse cancer morphology and cell phenotypes in FFPE sections.
对安装在载玻片上的福尔马林固定、石蜡包埋（FFPE）标本进行多重免疫荧光成像，可在保留空间和形态背景的同时识别多种细胞表型。多重免疫荧光方案已针对小鼠组织开发并验证。免疫表型分析可靠地描绘了癌症组织的免疫格局，已证明其会影响癌症的发生和发展，以及对治疗反应性和耐药性产生影响。在这里，我们描述了一种多重荧光图像分析方法，可对FFPE切片中的小鼠癌症形态和细胞表型进行分析。
